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Anti-S100 antibody [B32.1] - Astrocyte Marker (ab7852)

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Overview

Product name

Anti-S100 antibody [B32.1] - Astrocyte Marker
See all S100 products (24) ...

Description

Mouse monoclonal [B32.1] to S100 - Astrocyte Marker

Specificity

This antibody reacts with alpha and beta subunits of human S-100 protein (21-24 kD).

Tested applications

ICC/IF, IHC-P, IHC-FoFrmore details

Cross reactivity

Reacts with

Mouse, Rat, Cow, Human

Immunogen

Full length native protein (purified) (Cow).

Positive control

Melanoma tissues containing nerves, such as intestine and pancreas. Brain tissue.

Properties

Form

Liquid

Storage instructions

Store at +4°C. Do not freeze.

Storage buffer

pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: 0.01% PBS

Purity

Tissue culture supernatant

Clonality

Monoclonal

Clone number

B32.1

Isotype

IgG1

Light chain type

kappa

Research areas

Tags & Cell Markers >> Cell Type Markers >> Neuroscience Markers >> Glial
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Intermediate Filaments >> Class IV >> Neurofilaments
Neuroscience >> Cell Type Marker >> Glia marker >> Astrocyte marker
Neuroscience >> Neurotransmission >> Calcium Signaling >> Calcium Binding Proteins

  • Immunohistochemistry (PFA perfusion fixed frozen sections) - S100 antibody [B32.1] - Astrocyte Marker (ab7852)Immunohistochemistry (PFA perfusion fixed frozen sections) - S100 antibody [B32.1] - Astrocyte Marker (ab7852) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab7852 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide

    ICC/IF

     ICC/IF: 1/500 - 1/1000.(...Read more →

    ICC/IF: 1/500 - 1/1000.(PubMed: 19365568)

  • ShowHide1 Image

    IHC-P

     IHC-P: 1/50 - 1/100.(1/5...Read more →

    IHC-P: 1/50 - 1/100.(1/50 - 1/100. Prolonged fixation in buffered formalin can destroy the epitope.)

  • ShowHide1 Image

    IHC-FoFr

     IHC-FoFr: 1/500 - 1/1000...Read more →

    IHC-FoFr: 1/500 - 1/1000.(1/500 - 1/1000. Fix with 4% w/v paraformaldehyde and 15% saturated picric acid in 0.1 M. phosphate buffer pH 7.4.)

Target

Function

Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.

Tissue specificity

Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain.

Sequence similarities

Belongs to the S-100 family.
Contains 2 EF-hand domains.

Cellular localization

Cytoplasm.

Target information above from: UniProt accessionP23297 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Bpb antibody
  • NEF antibody
  • Protein S100-A1 antibody
  • S 100 protein alpha chain antibody
  • S 100 PROTEIN BETA CHAIN antibody
  • S 100 protein beta subunit antibody
  • S-100 protein alpha chain antibody
  • S-100 protein subunit alpha antibody
  • S100 alpha antibody
  • S100 beta antibody
  • S100 beta antibody
  • S100 calcium binding protein A1 antibody
  • S100 calcium binding protein B antibody
  • S100 calcium binding protein beta neural antibody
  • S100 calcium-binding protein A1 antibody
  • S100 protein alpha chain antibody
  • S100 protein alpha polypeptide antibody
  • S100 protein beta polypeptide neural antibody
  • S100A antibody
  • S100a1 antibody
  • S100B antibody
  • S100beta antibody
  • S10A1_HUMAN antibody
see all

Database links

see all

Anti-S100 antibody [B32.1] - Astrocyte Marker images:

  Immunohistochemistry (PFA perfusion fixed frozen sections) - S100 antibody [B32.1] - Astrocyte Marker (ab7852)

Immunohistochemistry (PFA perfusion fixed frozen sections) - S100 antibody [B32.1] - Astrocyte Marker (ab7852)

ab7852 at a dilution of 1/1000, staining Astrocytes (purple; Alexa 488 secondary at 1/2000) on 30µm coronal brain section tissue in free floating IHC (see protocol link for detailed description). [A] Image taken with 20x objective and [B] Image taken with 40x objective. Images coloured in Photoshop.

NB: No labeling observed following omission of primary antibody.

Sections were viewed using an Axioplan 2 Imaging microscope (Imaging Associates) fitted with 10x, 20x and 40x Plan-Neofluorobjectives (Zeiss, Germany) and images were taken using a AxioCam Hrm digital camera (Zeiss, Germany) and AxioVision software (Imaging Associates).

Protocols

  • IHC-FoFr protocol

    • References for Anti-S100 antibody [B32.1] - Astrocyte Marker (ab7852)

    This product has been referenced in:

    • Bax DAet al. Molecular and phenotypic characterisation of paediatric glioma cell lines as models for preclinical drug development. PLoS ONE 4:e5209 (2009). ICC/IF; Human.Read more (PubMed: 19365568) »
    • Nakagomi Tet al. Isolation and characterization of neural stem/progenitor cells from post-stroke cerebral cortex in mice. Eur J Neurosci 29:1842-52 (2009). IHC-FoFr; Mouse.Read more (PubMed: 19473237) »

    See all 7 publications for this product

    Publishing research using ab7852? Please let us know so that we can cite the reference in this datasheet

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"