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Immunohistochemistry (PFA perfusion fixed frozen sections) - S100 antibody [B32.1] - Astrocyte Marker (ab7852)
![Immunohistochemistry (PFA perfusion fixed frozen sections) - S100 antibody [B32.1] - Astrocyte Marker (ab7852)](/ps/datasheet/images/ab7852_1.jpg)
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Product Name
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S100 antibody [B32.1] - Astrocyte Marker
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See all S100 antibodies (23)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [B32.1] to S100 - Astrocyte Marker
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Immunogen
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Full length native protein (purified) (Cow).
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Reacts with
(species key)
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Hu, Ms, Rat, Cow, Pig
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Specificity
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This antibody reacts with alpha and beta subunits of human S-100 protein (21-24 kD).
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Tested applications
(see key)
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ICC/IF, IHC-FoFr, IHC-P, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: 1/500 - 1/1000.
IHC-P: 1/50 - 1/100. Prolonged fixation in buffered formalin can destroy the epitope.
IHC-FoFr: 1/500 - 1/1000. Fix with 4% w/v paraformaldehyde and 15% saturated picric acid in 0.1 M. phosphate buffer pH 7.4 (See recommended protocol).
WB: Use at an assay dependent dilution. Predicted molecular weight: 21-24 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Melanoma tissues containing nerves, such as intestine and pancreas. Brain tissue.
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Cellular localization
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Cytoplasmic and Nuclear
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Research areas
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Tags & Cell Markers >> Cell Type Markers >> Neuroscience Markers >> Glial
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Intermediate Filaments >> Class IV >> Neurofilaments
Neuroscience >> Cell Type Marker >> Glia marker >> Astrocyte marker
Neuroscience >> Neurotransmission >> Calcium Signaling >> Calcium Binding Proteins
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Relevance
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S100 belongs to the family of calcium binding proteins such as calmodulin and troponin C. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. S100A is composed of an alpha and beta chain whereas S100B is composed of two beta chains. S100 protein may function in stimulation of Ca2+ induced Ca2+ release, inhibition of microtubule assembly, and inhibition of protein kinase C mediated phosphorylation. Reduced expression of this protein has been implicated in cardiomyopathies. Immunoreactive S100 protein localizes in the cytoplasm and nuclei of astrocytes, Schwann's cells, ependymomas and astrogliomas. Ganglion cells do not stain for S100 protein. S100 can be detected in almost all benign naevi and malignant melanocytic tumours of the skin, and in Langerhans cells in the skin.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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B32.1
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Isotype
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IgG1
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Light chain type
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kappa
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Purity
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Tissue culture supernatant
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Storage buffer
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20 mM tris-borate, 150 mM sodium chloride,0.1% sodium azide Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.400 mg/ml
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Storage instructions
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Store at +4°C. Do not freeze.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this S100 antibody [B32.1] - Astrocyte Marker is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for S100 antibody [B32.1] - Astrocyte Marker images, references, products related to ab7852 and other tools.
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S100 antibody [B32.1] - Astrocyte Marker images:
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 Immunohistochemistry (PFA perfusion fixed frozen sections) - S100 antibody [B32.1] - Astrocyte Marker (ab7852)
ab7852 at a dilution of 1/1000, staining Astrocytes (purple; Alexa 488 secondary at 1/2000) on 30µm coronal brain section tissue in free floating IHC (see protocol link for detailed description). [A] Image taken with 20x objective and [B] Image taken with 40x objective. Images coloured in Photoshop.
NB: No labeling observed following omission of primary antibody.
Sections were viewed using an Axioplan 2 Imaging microscope (Imaging Associates) fitted with 10x, 20x and 40x Plan-Neofluorobjectives (Zeiss, Germany) and images were taken using a AxioCam Hrm digital camera (Zeiss, Germany) and AxioVision software (Imaging Associates).
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References for S100 antibody [B32.1] - Astrocyte Marker (ab7852)
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This product has been used in: (two most recent references)
Bax DA et al. Molecular and phenotypic characterisation of paediatric glioma cell lines as models for preclinical drug development. PLoS ONE 4:e5209 (2009). ICC/IF; Human. PubMed: 19365568
Nakagomi T et al. Isolation and characterization of neural stem/progenitor cells from post-stroke cerebral cortex in mice. Eur J Neurosci 29:1842-52 (2009). IHC-FoFr; Mouse. PubMed: 19473237
See all 7 publication references for this product.
If you publish research using ab7852 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to S100
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S100 antibody [B32.1] - Astrocyte Marker - more information
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S100 antibody [B32.1] - Astrocyte Marker - related products:
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S100 antibody [B32.1] - Astrocyte Marker - other tools:
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Immunohistochemistry (PFA perfusion fixed frozen sections) - S100 antibody [B32.1] - Astrocyte Marker (ab7852)](/index.html?datasheet=7852#ab7852_1.jpg)
