S100 beta antibody [EP1576Y] (ab52642)

Product Name 

S100 beta antibody [EP1576Y]

Product type 

Primary antibodies

Description 

Rabbit monoclonal [EP1576Y] to S100 beta

Immunogen 

Synthetic peptide corresponding to residues on the C-terminus of human S100 beta.

Reacts with 
(species key)

Hu, Ms, Rat

Tested applications 
(see key)

IHC-P, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
IHC-P: 1/100 - 1/250. We strongly recommend that customers perform an antigen retrieval step.
IP: 1/40.
WB: 1/1000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).

Is unsuitable for Flow Cyt.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

A375 cell lysate or human melanoma tissue.

Cellular localization 

Cytoplasmic and Nuclear

Research areas 

Stem Cells >> Neural Stem Cells >> Glial Restricted Lineage >> Astrocyte
Chromatin and Nuclear Signaling >> Cell cycle >> Cell Differentiation
Cell Biology >> Cell Cycle >> Cell differentiation

Relevance 

S100 beta is a member of the S100 family of proteins containing 2 EF-hand calcium binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 21q22.3. This protein may function in neurite extension, proliferation of melanoma cells, stimulation of Ca²⁺ fluxes, inhibition of PKC mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. Chromosomal rearrangements and altered expression of this gene have been implicated in several neurological, neoplastic, and other types of diseases, including Alzheimer's disease, Down's syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes.

Database links 

Raised in 

Rabbit

Clonality 

Monoclonal

Clone number 

EP1576Y

Isotype 

IgG

Purity 

Tissue culture supernatant

Storage buffer 

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Store at -20°C. Stable for 12 months at -20°C

Notes 

Produced under U.S. Patent No. 5,675,063.

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References for S100 beta antibody [EP1576Y] (ab52642)

  This product has been used in: (two most recent references)

Shi M et al. Identification of glutathione S-transferase pi as a protein involved in Parkinson disease progression. Am J Pathol 175:54-65 (2009). WB; Human. PubMed: 19498008

Buhimschi CS et al. Characterization of RAGE, HMGB1, and S100beta in inflammation-induced preterm birth and fetal tissue injury. Am J Pathol 175:958-75 (2009). IHC-P; Mouse. PubMed: 19679874
If you publish research using ab52642 please let us know so that we can cite the reference on this datasheet.

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