![Western blot - S100 beta antibody [EP1576Y] (ab52642) image](#ab52642_1.bmp)
![Immunohistochemistry (Paraffin-embedded sections) - S100 beta antibody [EP1576Y] (ab52642) image](#ab52642_2.bmp)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - S100 beta antibody [EP1576Y] (ab52642) image](#ab52642_1.jpg)
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Products:Cell Biology >> Cell Cycle >> Cell differentiation
Overview
Product name
Anti-S100 beta antibody [EP1576Y]
See all S100 beta products (13) ...
Description
Rabbit monoclonal [EP1576Y] to S100 beta
Tested applications
IHC-P, ICC/IF, WB, IP, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Human, Macaque Monkey
Immunogen
Synthetic peptide corresponding to residues on the C-terminus of human S100 beta.
Positive control
A375 cell lysate or human melanoma tissue.
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EP1576Y
Isotype
IgG
Research areas
Stem Cells >> Neural Stem Cells >> Glial Restricted Lineage >> Astrocyte
Epigenetics and Nuclear Signaling >> Cell cycle >> Cell Differentiation
Cell Biology >> Cell Cycle >> Cell differentiation
Applications
Show applications key
Our Abpromise guarantee covers the use of ab52642 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at an assay dependent dilution. (Antigen retrieval is strongly recommended.)
ICC/IF: Use at an assay dependent dilution. (PubMed: 20649577)
WB: 1/1000Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).
IP
IP: 1/40IP: 1/40
IHC-P: 1/100 - 1/250.(We strongly recommend that customers perform an antigen retrieval step.)
Application notes
Is unsuitable for or Flow Cyt.
Target
Function
Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling.
Tissue specificity
Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues.
Sequence similarities
Belongs to the S-101 family.
Contains 2 EF-hand domains.
Cellular localization
Cytoplasm. Nucleus.
Target information above from: UniProt accessionP04271
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- NEF antibody
- Protein S100 B antibody
- Protein S100-B antibody
see all
Database links
- Entrez Gene: 6285 Human
- Entrez Gene: 20203 Mouse
- Entrez Gene: 25742 Rat
- Omim: 176990 Human
- SwissProt: P04271 Human
- SwissProt: P50114 Mouse
- SwissProt: P04631 Rat
- Unigene: 422181 Human
see all
Anti-S100 beta antibody [EP1576Y] images:
Western blot - S100 beta antibody [EP1576Y] (ab52642)
![Western blot - S100 beta antibody [EP1576Y] (ab52642)](/ps/datasheet/Images/52/ab52642/ab52642_1.bmp)
Anti-S100 beta antibody [EP1576Y] (ab52642) at 1/1000 dilution + A375 cell lysate at 10 µg
Secondary
Goat anti-Rabbit HRP labeled at 1/2000 dilution
Predicted band size : 11 kDa
Observed band size : 11 kDa
Immunohistochemistry (Paraffin-embedded sections) - S100 beta antibody [EP1576Y] (ab52642)
![Immunohistochemistry (Paraffin-embedded sections) - S100 beta antibody [EP1576Y] (ab52642)](/ps/datasheet/Images/52/ab52642/ab52642_2.bmp)
Ab52642 (1/100) staining human S100 beta in human melanoma tissue by immunohistochemistry using paraffin embedded tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - S100 beta antibody [EP1576Y] (ab52642)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - S100 beta antibody [EP1576Y] (ab52642)](/ps/datasheet/Images/52/ab52642/ab52642_1.jpg)
ab52642 at 1/500 staining human melanoma tissue sections by IHC-P. The tissue was formladehyde fixed and a heat mediated antigen retrieval step was performed, before being blocked and incubated with the antibody for 1 hour. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
This image is courtesy of an Abreview submitted by Mrs Melissa Downing
References for Anti-S100 beta antibody [EP1576Y] (ab52642)
This product has been referenced in:
- Lu Jet al. EFFECT OF BLAST EXPOSURE ON THE BRAIN STRUCTURE AND COGNITION IN THE MACACA FASCICULARIS. J Neurotrauma : (2011). IHC-P; Macaque Monkey.Read more (PubMed: 21639720) »
- Selvadurai HJ & Mason JO Wnt/ß-catenin signalling is active in a highly dynamic pattern during development of the mouse cerebellum. PLoS One 6:e23012 (2011). IHC-P; Mouse.Read more (PubMed: 21857982) »
See all 8 publications for this product
Publishing research using ab52642? Please let us know so that we can cite the reference in this datasheet
![S100 beta antibody [EP1576Y] for IHC-P in Human (52642)](/ps/Reviews/Images/ab8220_35922.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"