S100 beta antibody [SH-B1] (ab11178)

Product Name 

S100 beta antibody [SH-B1]

Product type 

Primary antibodies

Description 

Mouse monoclonal [SH-B1] to S100 beta

Immunogen 

Full length native protein (purified) (Cow).

Reacts with 
(species key)

Hu, Rat, Cat, Cow, Pig, Rb

Specificity 

The product does not react with other members of the EF-hand family such as calmodulin, parvalbumin, intestinal calcium binding protein, and myosin light chain. The antibody should recognise S100a and S100b but not S100ao.

Tested applications 
(see key)

Dot, ELISA, ICC/IF, IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
Dot: Use at an assay dependent dilution.
ELISA: Use at an assay dependent dilution.
ICC/IF: 1/1000 (See Abreview).
IHC-P: 1/1000. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
WB: Use at an assay dependent dilution. Predicted molecular weight: 10.5 kDa.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Human tongue tissue slide (IHC-P).

Cellular localization 

Cytoplasmic and Nuclear

Research areas 

Stem Cells >> Neural Stem Cells >> Glial Restricted Lineage >> Astrocyte
Chromatin and Nuclear Signaling >> Cell cycle >> Cell Differentiation
Cell Biology >> Cell Cycle >> Cell differentiation

Relevance 

S100 beta is a member of the S100 family of proteins containing 2 EF-hand calcium binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 21q22.3. This protein may function in neurite extension, proliferation of melanoma cells, stimulation of Ca²⁺ fluxes, inhibition of PKC mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. Chromosomal rearrangements and altered expression of this gene have been implicated in several neurological, neoplastic, and other types of diseases, including Alzheimer's disease, Down's syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

SH-B1

Isotype 

IgG1

Purity 

Ascites

Storage buffer 

Preservative: 15mM Sodium Azide

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

Batch dependent within range: 9.90 - 9.90 mg/ml

Concentration of lot no.     is  

Concentration not available for this lot.

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Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

References for S100 beta antibody [SH-B1] (ab11178)

  This product has been used in: (two most recent references)

Qu Q et al. Orphan nuclear receptor TLX activates Wnt/beta-catenin signalling to stimulate neural stem cell proliferation and self-renewal. Nat Cell Biol 12:31-40; sup pp 1-9 (2010). PubMed: 20010817

Esposito G et al. Opposing control of cannabinoid receptor stimulation on amyloid-beta-induced reactive gliosis: in vitro and in vivo evidence. J Pharmacol Exp Ther 322:1144-52 (2007). PubMed: 17545311
If you publish research using ab11178 please let us know so that we can cite the reference on this datasheet.

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