![Western blot - S100A10 antibody [4E7E10] (ab52272) image](#S100A10-Primary-antibodies-ab52272-1.jpg)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - S100A10 antibody [4E7E10] (ab52272) image](#S100A10-Primary-antibodies-ab52272-2.jpg)
![Immunocytochemistry/ Immunofluorescence - S100A10 antibody [4E7E10] (ab52272) image](#S100A10-Primary-antibodies-ab52272-3.jpg)
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Products:Neuroscience >> Neurotransmission >> Calcium Signaling >> Calcium Binding Proteins
Overview
Product name
Anti-S100A10 antibody [4E7E10]
See all S100A10 products (6) ...
Description
Mouse monoclonal [4E7E10] to S100A10
Tested applications
WB, ICC/IF, IHC-P, IP, ELISAmore details
Cross reactivity
Reacts with
Human
Immunogen
Ni-NTA purified truncated recombinant S100A10 (Human) expressed in E. Coli
Positive control
Ethanol fixed L-02 cells
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Concentration
Concentration information loading...
Purity
Ascites
Clonality
Monoclonal
Clone number
4E7E10
Isotype
IgG1
Applications
Show applications key
Our Abpromise guarantee covers the use of ab52272 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution. Predicted molecular weight: 11 kDa.
ICC/IF: 1/100 - 1/500.
IHC-P: 1/200 - 1/1000.
IP: Use at an assay dependent dilution. (PubMed: 21372205)
ELISA
ELISA: 1/10000ELISA: 1/10000
Target
Function
Because S100A10 induces the dimerization of ANXA2/p36, it may function as a regulator of protein phosphorylation in that the ANXA2 monomer is the preferred target (in vitro) of tyrosine-specific kinase.
Sequence similarities
Belongs to the S-100 family.
Target information above from: UniProt accessionP60903
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 42C antibody
- AA409961 antibody
- AL024248 antibody
see all
Database links
- Entrez Gene: 6281 Human
- Omim: 114085 Human
- SwissProt: P60903 Human
- Unigene: 143873 Human
Anti-S100A10 antibody [4E7E10] images:
Western blot - S100A10 antibody [4E7E10] (ab52272)
![Western blot - S100A10 antibody [4E7E10] (ab52272)](/ps/datasheet/images/52/ab52272/S100A10-Primary-antibodies-ab52272-1.jpg)
All lanes : Anti-S100A10 antibody [4E7E10] (ab52272) at 1/2000 dilution
Lane 1 : Cell lysates prepared from MCF-7 cells
Lane 2 : Cell lysates prepared from HepG2 cells
Lane 3 : Cell lysates prepared from Hela cells
Lysates/proteins at 100 µg per lane.
Secondary
HRP-conjugated Goat polyclonal to mouse IgG
Predicted band size : 11 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - S100A10 antibody [4E7E10] (ab52272)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - S100A10 antibody [4E7E10] (ab52272)](/ps/datasheet/images/52/ab52272/S100A10-Primary-antibodies-ab52272-2.jpg)
ab52272 at 1/1000 dilution staining S100A10 in human brain tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). The image show positive staining in nerve and ganglion cells with ab52272.
Immunocytochemistry/ Immunofluorescence - S100A10 antibody [4E7E10] (ab52272)
![Immunocytochemistry/ Immunofluorescence - S100A10 antibody [4E7E10] (ab52272)](/ps/datasheet/images/52/ab52272/S100A10-Primary-antibodies-ab52272-3.jpg)
ab52272 at 1/500 dilution staining S100A10 in human Hela (left) and L-02 (right) cells by immunocytochemistry/ Immunofluorescence. An Alexa Fluor® 488 conjugated Goat polyclonal to mouse IgG was used as secondary antibody. Image show green staining with primary antiobody whilst actin filaments were stained red with DY-554 Phalloidin. Nuclei were stained blue using DRAQ5 fluorescent DNA dye.
References for Anti-S100A10 antibody [4E7E10] (ab52272)
This product has been referenced in:
- Celma CC & Roy P Interaction of Calpactin Light Chain (S100A10/p11) and a Viral NS Protein Is Essential for Intracellular Trafficking of Nonenveloped Bluetongue Virus. J Virol 85:4783-91 (2011).Read more (PubMed: 21411520) »
- Yang Xet al. DLC1 interaction with S100A10 mediates inhibition of in vitro cell invasion and tumorigenicity of lung cancer cells through a RhoGAP-independent mechanism. Cancer Res 71:2916-25 (2011). IP; Human.Read more (PubMed: 21372205) »
See all 2 publications for this product
Publishing research using ab52272? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"