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Anti-S100A10 antibody [EPR3317] (ab76472)

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Overview

Product name

Anti-S100A10 antibody [EPR3317]
See all S100A10 products (6) ...

Description

Rabbit monoclonal [EPR3317] to S100A10

Tested applications

WB, IHC-P, ICC, Flow Cytmore details

Cross reactivity

Reacts with

Human

Does not react with

Mouse, Rat

Immunogen

A synthetic peptide corresponding to residues in human S100A10.

Positive control

HaCaT, A431 and HT-29 cell lysates. Human colon tissue and human kidney tissue.

General notes

Produced under U.S. Patent No. 5,675,063.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, Tissue culture supernatant, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4

Purity

Tissue culture supernatant

Clonality

Monoclonal

Clone number

EPR3317

Isotype

IgG

Research areas

Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calcium Binding Proteins
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Annexins

  • Western blot - S100A10 antibody [EPR3317] (ab76472)Western blot - S100A10 antibody [EPR3317] (ab76472) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - S100A10 antibody [EPR3317] (ab76472)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - S100A10 antibody [EPR3317] (ab76472) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - S100A10 antibody [EPR3317] (ab76472)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - S100A10 antibody [EPR3317] (ab76472) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab76472 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: 1/5000 - 1/20000.Pre...Read more →

    WB: 1/5000 - 1/20000.Predicted molecular weight: 11 kDa.

  • ShowHide2 Images

    IHC-P

     IHC-P: 1/100 - 1/250.Per...Read more →

    IHC-P: 1/100 - 1/250.Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • ICC

     ICC: 1/50 - 1/100.

    ICC: 1/50 - 1/100.

  • Flow Cyt

     Flow Cyt: 1/50

    Flow Cyt: 1/50

Application notes

Is unsuitable for or IP.

Target

Function

Because S100A10 induces the dimerization of ANXA2/p36, it may function as a regulator of protein phosphorylation in that the ANXA2 monomer is the preferred target (in vitro) of tyrosine-specific kinase.

Sequence similarities

Belongs to the S-100 family.

Target information above from: UniProt accessionP60903 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • 42C antibody
  • AA409961 antibody
  • AL024248 antibody
  • Annexin II ligand antibody
  • Annexin II ligand, calpactin I, light polypeptide antibody
  • Annexin II, light chain antibody
  • ANX2L antibody
  • ANX2LG antibody
  • Ca[1] antibody
  • CAL12 antibody
  • CAL1L antibody
  • Calpactin I light chain antibody
  • Calpactin I, p11 subunit antibody
  • Calpactin-1 light chain antibody
  • Cellular ligand of annexin II antibody
  • CLP11 antibody
  • GP11 antibody
  • MGC111133 antibody
  • Nerve growth factor-induced protein 42C antibody
  • OTTHUMP00000015269 antibody
  • OTTHUMP00000015270 antibody
  • p10 antibody
  • p10 protein antibody
  • P11 antibody
  • Protein S100 A10 antibody
  • Protein S100-A10 antibody
  • S100 calcium binding protein A10 (annexin II ligand, calpactin I, light polypeptide (p11)) antibody
  • S100 calcium binding protein A10 (calpactin) antibody
  • S100 calcium binding protein A10 antibody
  • S100 calcium-binding protein A10 antibody
  • S100a10 antibody
  • S10AA_HUMAN antibody
see all

Database links

Anti-S100A10 antibody [EPR3317] images:

  Western blot - S100A10 antibody [EPR3317] (ab76472)

Western blot - S100A10 antibody [EPR3317] (ab76472)

All lanes : Anti-S100A10 antibody [EPR3317] (ab76472) at 1/10000 dilution

Lane 1 : HaCaT cell lysate
Lane 2 : A431 cell lysate
Lane 3 : HT-29 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
HRP labelled goat anti-rabbit at 1/1000 dilution

Predicted band size : 11 kDa
Observed band size : 11 kDa

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - S100A10 antibody [EPR3317] (ab76472)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - S100A10 antibody [EPR3317] (ab76472)

ab76472 at 1/100 dilution staining S100A10 in human colon tissue.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - S100A10 antibody [EPR3317] (ab76472)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - S100A10 antibody [EPR3317] (ab76472)

ab76472 at 1/100 dilution staining S100A10 in human kidney tissue.

References for Anti-S100A10 antibody [EPR3317] (ab76472)

ab76472 has not yet been referenced specifically in any publications.

Publishing research using ab76472? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"