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Western blot - S100A10 antibody [EPR3317] (ab76472)
![Western blot - S100A10 antibody [EPR3317] (ab76472)](/ps/datasheet/images/ab76472_1.jpg)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - S100A10 antibody [EPR3317] (ab76472)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - S100A10 antibody [EPR3317] (ab76472)](/ps/datasheet/images/ab76472_2.gif)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - S100A10 antibody [EPR3317] (ab76472)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - S100A10 antibody [EPR3317] (ab76472)](/ps/datasheet/images/ab76472_4.gif)
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Product Name
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S100A10 antibody [EPR3317]
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See all S100A10 antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rabbit monoclonal [EPR3317] to S100A10
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Immunogen
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A synthetic peptide corresponding to residues in human S100A10.
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Reacts with
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Hu
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Does not react with
(species key)
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Ms, Rat
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Tested applications
(see key)
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Flow Cyt, ICC, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Flow Cyt: 1/50.
ICC: 1/50 - 1/100.
IHC-P: 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB: 1/5000 - 1/20000. Predicted molecular weight: 11 kDa.
Is unsuitable for IP.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HaCaT, A431 and HT-29 cell lysates. Human colon tissue and human kidney tissue.
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Cellular localization
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Cytoplasmic
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Research areas
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Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calcium Binding Proteins
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Annexins
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Relevance
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The S100 family of proteins contain two EF-hand calcium-binding motifs and are thought to be involved in the regulation of a number of cellular processes including cell cycle progression and differentiation.
S100A10 induces the dimerization of ANXA2/p36, it may function as a regulator of protein phosphorylation in that the ANXA2 monomer is the preferred target (in vitro) of tyrosine-specific kinase.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Monoclonal
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Clone number
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EPR3317
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Isotype
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IgG
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Purity
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Tissue culture supernatant
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Storage buffer
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Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, Tissue culture supernatant, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Notes
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Produced under U.S. Patent No. 5,675,063.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this S100A10 antibody [EPR3317] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for S100A10 antibody [EPR3317] images, references, products related to ab76472 and other tools.
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S100A10 antibody [EPR3317] images:
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 Western blot - S100A10 antibody [EPR3317] (ab76472)
All lanes : S100A10 antibody [EPR3317] (ab76472) at 1/10000 dilution
Lane 1 : HaCaT cell lysate
Lane 2 : A431 cell lysate
Lane 3 : HT-29 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
HRP labelled goat anti-rabbit at 1/1000 dilution
Predicted band size : 11 kDa
Observed band size : 11 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - S100A10 antibody [EPR3317] (ab76472)
ab76472 at 1/100 dilution staining S100A10 in human colon tissue.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - S100A10 antibody [EPR3317] (ab76472)
ab76472 at 1/100 dilution staining S100A10 in human kidney tissue.
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Search PubMed (MEDLINE) for references to S100A10
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S100A10 antibody [EPR3317] - more information
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Customer reviews (feedback) regarding S100A10 antibody [EPR3317] |
Customer FAQs regarding S100A10 antibody [EPR3317] |
Protocols for S100A10 antibody [EPR3317] |
Price and availability of products related to S100A10 antibody [EPR3317] |
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S100A10 antibody [EPR3317] - related products:
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S100A10 antibody [EPR3317] - other tools:
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Contact Abcam with a Technical Enquiry about S100A10 antibody [EPR3317] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
![Western blot - S100A10 antibody [EPR3317] (ab76472)](/index.html?datasheet=76472#ab76472_1.jpg)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - S100A10 antibody [EPR3317] (ab76472)](/index.html?datasheet=76472#ab76472_2.gif)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - S100A10 antibody [EPR3317] (ab76472)](/index.html?datasheet=76472#ab76472_4.gif)
