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Western blot - S100A12 antibody (ab37657)
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Immunohistochemistry (Frozen sections) - S100A12 antibody (ab37657)
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Product Name
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S100A12 antibody
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See all S100A12 antibodies (2)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to S100A12
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Immunogen
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Recombinant S100A12 (Human) with 6x His tag
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ELISA, IHC-Fr, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/2000 - 1/10000.
IHC-Fr: 1/2000.
WB: 1/1000 - 1/10000. Detects a band of approximately 10.5 kDa (predicted molecular weight: 10.5 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Secreted
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Research areas
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Immunology >> Secreted Molecules >> Other secreted molecules
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calcium Binding Proteins
Immunology >> Innate Immunity >> Cytokines >> Other
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Relevance
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S100 family proteins are acidic low molecular weight proteins containing 2 EF-hand calcium binding domains. They are characterised by their cell-type specific expression patterns. S100A12 is a member of the calgranulin sub-family. Calgranulins are S100 proteins that are expressed by neutrophils and also found in infiltrating monocytes and granulocytes during chronic inflammation. S100A12 is expressed in neutrophils and monocytes. It is highly abundant in resting neutrophils, comprising 5% of total cytostolic protein.
S100A12 is a ligand for RAGE. Interaction with RAGE on mononuclear phagocytes, lymphocytes and endothelium triggers cellular activation. Nuclear factor kappa B, a key transcription factor for inflammatory events, is activated by S100A12/RAGE.
S100A12 has also been shown to have anti-microbial properties.
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S100A12 antibody (ab37657) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.1% Sodium Azide
Constituents: PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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2.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this S100A12 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for S100A12 antibody images, references, products related to ab37657 and other tools.
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S100A12 antibody images:
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 Western blot - S100A12 antibody (ab37657)
S100A12 antibody (ab37657) + 200 ng recombinant human S100A12
Secondary
anti-rabbit IgG alkalind phosphatase conjugate
Predicted band size : 10.5 kDa
Observed band size : 10.5 kDa
Additional bands at : 21 kDa (possible dimer).
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Immunohistochemistry (Frozen sections) - S100A12 antibody (ab37657)
Anti-S100A12 antibody (ab37657) detects macrophage infiltrates expressing S100A12
in colon tissues from a patient with Crohn’s disease.
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References for S100A12 antibody (ab37657)
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This product has been used in: (two most recent references)
Chung L et al. Proteomic profiling of growth hormone-responsive proteins in human peripheral blood leukocytes. J Clin Endocrinol Metab 94:3038-43 (2009). WB; Human. PubMed: 19491220
Srikrishna G et al. Carboxylated glycans mediate colitis through activation of NF-kappa B. J Immunol 175:5412-22 (2005). PubMed: 16210648
If you publish research using ab37657 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to S100A12
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S100A12 antibody - more information
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Customer reviews (feedback) regarding S100A12 antibody |
Customer FAQs regarding S100A12 antibody |
Protocols for S100A12 antibody |
Price and availability of products related to S100A12 antibody |
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S100A12 antibody - related products:
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S100A12 antibody - other tools:
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Contact Abcam with a Technical Enquiry about S100A12 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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