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If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab68124? Please let us know so that we can cite the reference in this datasheet
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
![Flow Cytometry - S100A4 antibody [NJ4F3] (ab68124)](/ps/datasheet/Images/68/ab68124/ab68124facs.jpg)
Flow Cytometry - S100A4 antibody [NJ4F3] (ab68124)
FACS analysis of BOSC23 cells, using ab68124 at 1.2µg for 106 cells. BOSC23 cells were transiently transfected with an expression vector encoding either S100 A4 (red curve) or an irrelevant protein (control transfectant: black curve). Binding of NJ-4F3 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with S100 A4 transfected cells.
![SDS-PAGE - S100A4 antibody [NJ4F3] (ab68124)](/ps/datasheet/Images/68/ab68124/ab68124sdspage.jpg)
SDS-PAGE - S100A4 antibody [NJ4F3] (ab68124)
SDS-PAGE analysis of purified NJ-4F3 monoclonal antibody.
Lane 1: Molecular weight marker
Lane 2: 2 µg of purified NJ-4F3 monoclonal antibody.
Proteins were separated by capillary gel electrophoresis.
Internal control bands: 240 kDa, 7 kDa and 4.5 kDa.
](/ps/datasheet/images/68/ab68124/S100A4-Primary-antibodies-ab68124-1.jpg)
Immunocytochemistry/ Immunofluorescence-S100A4 antibody [NJ4F3](ab68124)
ICC/IF image of ab68124 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab68124, 5µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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