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Flow Cytometry - S100A4 antibody [NJ4F3] (ab68124)
![Flow Cytometry - S100A4 antibody [NJ4F3] (ab68124)](/ps/datasheet/images/ab68124facs.jpg)
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SDS-PAGE - S100A4 antibody [NJ4F3] (ab68124)
![SDS-PAGE - S100A4 antibody [NJ4F3] (ab68124)](/ps/datasheet/images/ab68124sdspage.jpg)
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Product Name
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S100A4 antibody [NJ4F3]
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See all S100A4 antibodies (7)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [NJ4F3] to S100A4
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Immunogen
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Full length native protein (purified)
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ELISA, Flow Cyt, cELISA
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Abreviews
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Application notes
(see key)
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Recommended dilutions
cELISA: 1/200 - 1/400.
ELISA: 1/200 - 1/400.
Flow Cyt: Use 1.2µg for 106 cells.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cytoplasmic and Nuclear
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Research areas
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Cardiovascular >> Heart >> Cardiogenesis >> Myocardial regeneration
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> Other
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calcium Binding Proteins
Cardiovascular >> Angiogenesis >> Angiogenic Factors
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Relevance
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S100 belongs to the family of calcium binding proteins such as calmodulin and troponin C. S100A is composed of an alpha and beta chain whereas S100B is composed of two beta chains. S100 protein is also expressed in the antigen presenting cells such as the Langerhans cells in skin and interdigitating reticulum cells in the paracortex of lymph nodes. S100A4 has been implicated in motility, invasion and tubulin polymerization. It is also involved in tumor metastasis.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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NJ4F3
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Isotype
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IgG1
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Purity
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Protein G purified
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Storage buffer
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Preservative: None
Constituents: PBS, pH 7.2
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Form
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Liquid
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Concentration
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1.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this S100A4 antibody [NJ4F3] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for S100A4 antibody [NJ4F3] images, references, products related to ab68124 and other tools.
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S100A4 antibody [NJ4F3] images:
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 Flow Cytometry - S100A4 antibody [NJ4F3] (ab68124)
FACS analysis of BOSC23 cells, using ab68124 at 1.2µg for 106 cells. BOSC23 cells were transiently transfected with an expression vector encoding either S100 A4 (red curve) or an irrelevant protein (control transfectant: black curve). Binding of NJ-4F3 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with S100 A4 transfected cells.
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SDS-PAGE - S100A4 antibody [NJ4F3] (ab68124)
SDS-PAGE analysis of purified NJ-4F3 monoclonal antibody.
Lane 1: Molecular weight marker
Lane 2: 2 µg of purified NJ-4F3 monoclonal antibody.
Proteins were separated by capillary gel electrophoresis.
Internal control bands: 240 kDa, 7 kDa and 4.5 kDa.
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Search PubMed (MEDLINE) for references to S100A4
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S100A4 antibody [NJ4F3] - more information
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Customer reviews (feedback) regarding S100A4 antibody [NJ4F3] |
Customer FAQs regarding S100A4 antibody [NJ4F3] |
Protocols for S100A4 antibody [NJ4F3] |
Price and availability of products related to S100A4 antibody [NJ4F3] |
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S100A4 antibody [NJ4F3] - related products:
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S100A4 antibody [NJ4F3] - other tools:
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Contact Abcam with a Technical Enquiry about S100A4 antibody [NJ4F3] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Flow Cytometry - S100A4 antibody [NJ4F3] (ab68124)](/index.html?datasheet=68124#ab68124facs.jpg)
![SDS-PAGE - S100A4 antibody [NJ4F3] (ab68124)](/index.html?datasheet=68124#ab68124sdspage.jpg)
