Anti-S100A4 antibody [NJ4F3] (ab68124)

  Flow Cytometry - S100A4 antibody [NJ4F3] (ab68124)

FACS analysis of BOSC23 cells, using ab68124 at 1.2µg for 10⁶ cells. BOSC23 cells were transiently transfected with an expression vector encoding either S100 A4 (red curve) or an irrelevant protein (control transfectant: black curve). Binding of NJ-4F3 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with S100 A4 transfected cells.

  SDS-PAGE - S100A4 antibody [NJ4F3] (ab68124)

SDS-PAGE analysis of purified NJ-4F3 monoclonal antibody.
Lane 1: Molecular weight marker
Lane 2: 2 µg of purified NJ-4F3 monoclonal antibody.
Proteins were separated by capillary gel electrophoresis.
Internal control bands: 240 kDa, 7 kDa and 4.5 kDa.

  Immunocytochemistry/ Immunofluorescence-S100A4 antibody [NJ4F3](ab68124)

ICC/IF image of ab68124 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab68124, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.