Anti-S100A4 antibody (ab27957)

  Immunocytochemistry/ Immunofluorescence - S100A4 antibody (ab27957)

ab27957 (1/200) detecting S100A4 in mouse neural stem cells (green). Cells were fixed in formaldehyde, permeabilized in 0.1% Triton and blocked in 8% BSA. Cells were counter stained with DAPI to visualize the nucleus (blue).

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  Immunohistochemistry (PFA perfusion fixed frozen sections) - S100A4 antibody (ab27957)

The thymi obtained from mice were flash-frozen and subsequently embedded in frozen section medium and cut into 5–10-µm-thick cryostat sections. At least three serial sections were utilized for each staining. Tissue sections were then fixed with 4% buffered paraformaldehyde and stained with various combinations of the following primary antibodies to mouse antigens: unconjugated rabbit polyclonal anti-aP2; unconjugated rabbit antibody to S100A4 (FSP1) (ab27957). Slides were incubated with Alexa-Fluor secondary regents. Nuclei were visualized with 4',6-diamidine-2'phenylindole dihydrochloride (DAPI).

Image shows the subcapsular region of thymus showing co-localization of ß-galactosidase (red), S100A4 (FSP1) (blue), and aP2 (green).

Image from Youm YH et al, J Biol Chem. 2009 Mar 13;284(11):7068-77, Fig 4.