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Products:Cardiovascular >> Angiogenesis >> Angiogenic Factors
Overview
Product name
Anti-S100A4 antibody
See all S100A4 products (11) ...
Description
Rabbit polyclonal to S100A4
Tested applications
WB, ICC/IF, IHC-Pmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Cow, Dog
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 50 to the C-terminus of Human S100A4.
(Peptide available as ab41530.)
Positive control
This antibody gives a positive signal in Human S100A4 Recombinant Protein.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Heart >> Cardiogenesis >> Myocardial regeneration
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> Other
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calcium Binding Proteins
Cardiovascular >> Angiogenesis >> Angiogenic Factors
Applications
Show applications key
Our Abpromise guarantee covers the use of ab40722 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 37 kDa (predicted molecular weight: 12 kDa).
ICC/IF: Use at an assay dependent dilution. (PubMed: 20551063)
IHC-P: Use at an assay dependent concentration.
Target
Tissue specificity
Ubiquitously expressed.
Sequence similarities
Belongs to the S-100 family.
Contains 2 EF-hand domains.
Target information above from: UniProt accessionP26447
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 18A2 antibody
- 42A antibody
- Calcium protein antibody
see all
Database links
- Entrez Gene: 282343 Cow
- Entrez Gene: 403787 Dog
- Entrez Gene: 6275 Human
- Omim: 114210 Human
- SwissProt: P35466 Cow
- SwissProt: Q3ZBN9 Cow
- SwissProt: Q9TV56 Dog
- SwissProt: P26447 Human
see all
Anti-S100A4 antibody images:
Western blot - S100A4 antibody (ab40722)
Anti-S100A4 antibody (ab40722) at 1 µg/ml + S100A4 - Recombinant Protein at 0.1 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 12 kDa
Observed band size : 37 kDa (why is the actual band size different from the predicted?)
The S100A4 tagged recombinant protein has a molecular weight of 36.8 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - S100A4 antibody (ab40722)
ab40722 staining S100A4 in human heart tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent fixation in formaldehyde, heat mediated antigen retrieval in Citrate buffer pH 6.0 and blocking (5 minutes/peroxidase block then 10 minutes/protein block) for 15 minutes at 20°C. The primary antibody was diluted, 1/250 and incubated with sample for 45 minutes at 20°C. A HRP conjugated goat polyclonal to rabbit IgG was used undiluted as secondary.
This image is courtesy of an Abreview submitted by Antibody Solutions Ltd.
References for Anti-S100A4 antibody (ab40722)
This product has been referenced in:
- Baljinnyam Eet al. Exchange Protein Directly Activated by Cyclic AMP Increases Melanoma Cell Migration by a Ca2+-Dependent Mechanism. Cancer Res 70:5607-17 (2010). ICC/IF; Human.Read more (PubMed: 20551063) »
- Das RKet al. Assessment of malignant potential of oral submucous fibrosis through evaluation of p63, E-cadherin and CD105 expression. J Clin Pathol 63:894-9 (2010). IHC-P; Human.Read more (PubMed: 20876321) »
See all 2 publications for this product
Publishing research using ab40722? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"