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ab41531 |
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ab85489 |
If your product does not perform as described on this datasheet, we will refund or replace your product...
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We got a new batch of ab68124 (S100A4 antibody [NJ4F3]andhttp://www.abcam.com/S100A4-antibody-ab41532.htmlhttp://www.abcam.com/S100A4-antibody-ab41532.htmlRabbit polyclonal) LOT-GR38457-1 this one dose not work in over hands, however, apreviouslotworked fine so I wonder if you have an old ore a suggestion on have to handle this problem, |
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ANSWER: |
According to our records, ab41532 was proving difficult to use and we were in contact in order to help resolve the issue. |
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Testing discount codes to try ab7311 & ab41532 |
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DISCOUNT CODE: *********** |
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We got a new batch of ab68124 (S100A4 antibody [NJ4F3]andhttp://www.abcam.com/S100A4-antibody-ab41532.htmlhttp://www.abcam.com/S100A4-antibody-ab41532.htmlRabbit polyclonal) LOT-GR38457-1 this one dose not work in over hands, however, apreviouslotworked fine so I wonder if you have an old ore a suggestion on have to handle this problem, |
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ANSWER: |
Thank you for contacting us. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Anti-S100A4 antibody (ab41532) at 1 µg/ml + S100A4 - Recombinant Protein at 0.1 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 12 kDa
Observed band size : 37 kDa (why is the actual band size different from the predicted?)
The S100A4 tagged recombinant protein has a molecular weight of 36.8 kDa.
ab41532 staining S100A4 in human heart tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent fixation in formaldehyde, heat mediated antigen retrieval in Citrate buffer pH 6.0 and blocking (5 minutes/peroxidase block then 10 minutes/protein block) for 15 minutes at 20°C. The primary antibody was diluted, 1/2000 and incubated with sample for 45 minutes at 20°C. A HRP conjugated goat polyclonal to rabbit IgG was used undiluted as secondary.
This image is courtesy of an Abreview submitted by Antibody Solutions Ltd.
ICC/IF image of ab41532 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab41532, 5µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa, Hek293 and MCF7 cells at 5µg/ml, and in 100% methanol fixed (5 min) HeLa, Hek293, HepG2 and MCF7 cells at 5µg/ml.
Anti-S100A4 antibody (ab41532) at 1/250 dilution +
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 20 minutes
1
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