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Western blot - S100A4 antibody (ab41532)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - S100A4 antibody (ab41532)
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Immunocytochemistry/ Immunofluorescence - S100A4 antibody (ab41532)
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Product Name
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S100A4 antibody
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See all S100A4 antibodies (7)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to S100A4
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human S100A4. (Peptide available as ab41531.)
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Reacts with
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Hu
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Predicted to react
(species key)
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Ms, Rat, Cow, Dog
(Due to sequence homology)
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Tested applications
(see key)
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ICC/IF, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 5 µg/ml.
IHC-P: Use at an assay dependent concentration.
WB: 1/250. Detects a band of approximately 37 kDa (predicted molecular weight: 12 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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This antibody gives a positive signal in Human S100A4 Recombinant Protein.
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Cellular localization
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Cytoplasmic and Nuclear
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Research areas
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Cardiovascular >> Heart >> Cardiogenesis >> Myocardial regeneration
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> Other
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calcium Binding Proteins
Cardiovascular >> Angiogenesis >> Angiogenic Factors
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Relevance
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S100 belongs to the family of calcium binding proteins such as calmodulin and troponin C. S100A is composed of an alpha and beta chain whereas S100B is composed of two beta chains. S100 protein is also expressed in the antigen presenting cells such as the Langerhans cells in skin and interdigitating reticulum cells in the paracortex of lymph nodes. S100A4 has been implicated in motility, invasion and tubulin polymerization. It is also involved in tumor metastasis.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Batch dependent within range: 0.70 - 0.70 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this S100A4 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for S100A4 antibody images, references, products related to ab41532 and other tools.
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S100A4 antibody images:
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 Western blot - S100A4 antibody (ab41532)
S100A4 antibody (ab41532) at 1 µg/ml + S100A4 - Recombinant Protein at 0.1 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 12 kDa
Observed band size : 37 kDa (why is the actual band size different from the predicted?)
The S100A4 tagged recombinant protein has a molecular weight of 36.8 kDa.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - S100A4 antibody (ab41532)
ab41532 staining S100A4 in human heart tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent fixation in formaldehyde, heat mediated antigen retrieval in Citrate buffer pH 6.0 and blocking (5 minutes/peroxidase block then 10 minutes/protein block) for 15 minutes at 20°C. The primary antibody was diluted, 1/2000 and incubated with sample for 45 minutes at 20°C. A HRP conjugated goat polyclonal to rabbit IgG was used undiluted as secondary. This image is courtesy of an Abreview submitted by Antibody Solutions Ltd.
See Abreview
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Immunocytochemistry/ Immunofluorescence - S100A4 antibody (ab41532)
ICC/IF image of ab41532 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab41532, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa, Hek293 and MCF7 cells at 5µg/ml, and in 100% methanol fixed (5 min) HeLa, Hek293, HepG2 and MCF7 cells at 5µg/ml.
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Search PubMed (MEDLINE) for references to S100A4
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S100A4 antibody - more information
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Customer reviews (feedback) regarding S100A4 antibody |
Customer FAQs regarding S100A4 antibody |
Protocols for S100A4 antibody |
Price and availability of products related to S100A4 antibody |
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S100A4 antibody - related products:
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S100A4 antibody - other tools:
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Contact Abcam with a Technical Enquiry about S100A4 antibody |
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