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Products:Cardiovascular >> Angiogenesis >> Angiogenic Factors
Overview
Product name
Anti-S100A4 antibody
See all S100A4 products (11) ...
Description
Rabbit polyclonal to S100A4
Tested applications
WB, IHC-P, ICC/IFmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Cow, Dog
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human S100A4.
(Peptide available as ab41531.)
Positive control
This antibody gives a positive signal in Human S100A4 Recombinant Protein.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Heart >> Cardiogenesis >> Myocardial regeneration
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> Other
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calcium Binding Proteins
Cardiovascular >> Angiogenesis >> Angiogenic Factors
Applications
Show applications key
Our Abpromise guarantee covers the use of ab41532 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/250Predicted molecular weight: 12 kDa.
IHC-P: Use at an assay dependent dilution.
ICC/IF: Use a concentration of 5 µg/ml
Target
Tissue specificity
Ubiquitously expressed.
Sequence similarities
Belongs to the S-100 family.
Contains 2 EF-hand domains.
Target information above from: UniProt accessionP26447
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 18A2 antibody
- 42A antibody
- Calcium protein antibody
see all
Database links
- Entrez Gene: 282343 Cow
- Entrez Gene: 403787 Dog
- Entrez Gene: 6275 Human
- Entrez Gene: 20198 Mouse
- Entrez Gene: 24615 Rat
- Omim: 114210 Human
- SwissProt: P35466 Cow
- SwissProt: Q3ZBN9 Cow
see all
Anti-S100A4 antibody images:
Western blot - S100A4 antibody (ab41532)
Anti-S100A4 antibody (ab41532) at 1 µg/ml + S100A4 - Recombinant Protein at 0.1 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 12 kDa
Observed band size : 37 kDa (why is the actual band size different from the predicted?)
The S100A4 tagged recombinant protein has a molecular weight of 36.8 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - S100A4 antibody (ab41532)
ab41532 staining S100A4 in human heart tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent fixation in formaldehyde, heat mediated antigen retrieval in Citrate buffer pH 6.0 and blocking (5 minutes/peroxidase block then 10 minutes/protein block) for 15 minutes at 20°C. The primary antibody was diluted, 1/2000 and incubated with sample for 45 minutes at 20°C. A HRP conjugated goat polyclonal to rabbit IgG was used undiluted as secondary.
This image is courtesy of an Abreview submitted by Antibody Solutions Ltd.
Immunocytochemistry/ Immunofluorescence - S100A4 antibody (ab41532)
ICC/IF image of ab41532 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab41532, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa, Hek293 and MCF7 cells at 5µg/ml, and in 100% methanol fixed (5 min) HeLa, Hek293, HepG2 and MCF7 cells at 5µg/ml.
Western blot - Anti-S100A4 antibody (ab41532)
Anti-S100A4 antibody (ab41532) at 1/250 dilution + S100A4 protein (Human) (ab83650) at 0.01 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 20 minutes
References for Anti-S100A4 antibody (ab41532)
This product has been referenced in:
- Emilie Bet al. The MUC4 membrane-bound mucin regulates esophageal cancer cell proliferation and migration properties: Implication for S100A4 protein. Biochem Biophys Res Commun : (2011). IHC-P; Human.Read more (PubMed: 21889495) »
See 1 publication for this product
Publishing research using ab41532? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"