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Western blot - S100A9 antibody (ab63818)
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Immunocytochemistry/ Immunofluorescence - S100A9 antibody (ab63818)
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Product Name
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S100A9 antibody
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See all S100A9 antibodies (10)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to S100A9
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human S100A9. (Peptide available as ab74434.)
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Reacts with
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Hu
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Predicted to react
(species key)
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Chmp
(Due to sequence homology)
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Tested applications
(see key)
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ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 5 µg/ml.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 13 kDa (predicted molecular weight: 13 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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This antibody gave a positive signal in the following Human Tissue Lysates:
Lymph node, Thymus, Spleen, Lung
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Cellular localization
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Cytoplasm; Nucleus (not nucleolus)
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Research areas
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Cancer >> Tumor biomarkers >> Other
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calcium Binding Proteins
Immunology >> Innate Immunity >> Macrophage / Inflamm.
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Relevance
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S100A9 is a member of the S100 family of proteins. S100A9, together with S100A8 forms a heterodimeric protein complex, Calprotectin, which is a major calcium- and zinc-binding protein in the cytosol of neutrophils, monocytes, and keratinocytes. Complexes of S100A8 and S100A9 are the physiologically relevant forms of these proteins.
S100A9 may function in the inhibition of casein kinase and altered expression of this protein is associated with the disease cystic fibrosis.
Its expression and potential cytokine-like function in inflammation and in cancer suggest that S100A8/A9 may play a key role in inflammation-associated cancer.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.300 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this S100A9 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for S100A9 antibody images, references, products related to ab63818 and other tools.
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S100A9 antibody images:
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 Immunocytochemistry/ Immunofluorescence - S100A9 antibody (ab63818)
ICC/IF image of ab63818 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab63818, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) HeLa, HepG2 and Hek293 cells at 5µg/ml, and in 4% PFA fixed (10 min) HeLa, HepG2, Hek293 and MCF7 cells at 5µg/ml.
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Search PubMed (MEDLINE) for references to S100A9
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S100A9 antibody - more information
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Customer reviews (feedback) regarding S100A9 antibody |
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