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Western blot - S100A9 antibody (ab81522)
(enlarge)
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Product Name
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S100A9 antibody
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See all S100A9 antibodies (10)...
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Product type
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Primary antibodies
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Description
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Goat polyclonal to S100A9
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Immunogen
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Synthetic peptide: C-DTNADKQLSFEEF, corresponding to internal sequence amino acids 67-79 of Human S100A9 according to NP_002956.1
BLAST 'C-DTNADKQLSFEEF' with  or 
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Reacts with
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Hu
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Predicted to react
(species key)
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Cow, Rb
(Due to sequence homology)
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Peptide ELISA: antibody detection limit dilution: 1/8000.
WB: Use at a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 13 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human Peripheral Mononucleocytes lysate
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Cellular localization
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Cytoplasm; Nucleus (not nucleolus)
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Research areas
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Cancer >> Tumor biomarkers >> Other
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calcium Binding Proteins
Immunology >> Innate Immunity >> Macrophage / Inflamm.
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Relevance
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S100A9 is a member of the S100 family of proteins. S100A9, together with S100A8 forms a heterodimeric protein complex, Calprotectin, which is a major calcium- and zinc-binding protein in the cytosol of neutrophils, monocytes, and keratinocytes. Complexes of S100A8 and S100A9 are the physiologically relevant forms of these proteins.
S100A9 may function in the inhibition of casein kinase and altered expression of this protein is associated with the disease cystic fibrosis.
Its expression and potential cytokine-like function in inflammation and in cancer suggest that S100A8/A9 may play a key role in inflammation-associated cancer.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris saline, pH 7.3 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this S100A9 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for S100A9 antibody images, references, products related to ab81522 and other tools.
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S100A9 antibody images:
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 Western blot - S100A9 antibody (ab81522)
S100A9 antibody (ab81522) at 0.5 µg/ml (for 1 hour) + Human Peripheral Mononucleocytes lysate in RIPA buffer at 35 µg
developed using the ECL technique
Predicted band size : 13 kDa
Observed band size : 16 kDa (why is the actual band size different from the predicted?)
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Search PubMed (MEDLINE) for references to S100A9
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S100A9 antibody - more information
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Customer reviews (feedback) regarding S100A9 antibody |
Customer FAQs regarding S100A9 antibody |
Protocols for S100A9 antibody |
Price and availability of products related to S100A9 antibody |
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S100A9 antibody - related products:
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S100A9 antibody - other tools:
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Contact Abcam with a Technical Enquiry about S100A9 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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