Recombinant Anti-S100P antibody [EPR6142] (ab124743)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6142] to S100P
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-S100P antibody [EPR6142]
See all S100P primary antibodies -
Description
Rabbit monoclonal [EPR6142] to S100P -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human S100P aa 1-100. The exact sequence is proprietary.
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Positive control
- BxPC3 cell lysate; Human pancreatic adenocarcinoma, placenta and spleen tissues. WB: HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6142 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124743 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
Use a concentration of 1 µg/ml.
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WB |
1/1000. Detects a band of approximately 10 kDa (predicted molecular weight: 10 kDa).
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IHC-P |
1/150 - 1/300. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ICC/IF |
1/150.
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Notes |
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Flow Cyt (Intra)
Use a concentration of 1 µg/ml. |
WB
1/1000. Detects a band of approximately 10 kDa (predicted molecular weight: 10 kDa). |
IHC-P
1/150 - 1/300. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ICC/IF
1/150. |
Target
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Tissue specificity
Up-regulated in various pancreatic ductal adenocarcinomas and pancreatic intraepithelial neoplasias. -
Sequence similarities
Belongs to the S-100 family.
Contains 2 EF-hand domains. -
Cellular localization
Nucleus. Cytoplasm. Colocalizes with S100PBP in the nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6286 Human
- Omim: 600614 Human
- SwissProt: P25815 Human
- Unigene: 2962 Human
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Alternative names
- MIG9 antibody
- Migration inducing gene 9 antibody
- Protein S100-E antibody
see all
Images
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ab124743 was shown to react with S100P in wild-type HeLa cells in western blot. Loss of signal was observed when S100P knockout sample was used. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab124743 overnight at 4 °C at a 1 in 1000 dilution. Blots were incubated with HRP conjugated Goat anti-Rabbit (H+L) secondary antibody at 1/5000 for 1 hour at room temperature before development with Optiblot ECL reagent (ab133456) and imaging.
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Anti-S100P antibody [EPR6142] (ab124743) at 1/1000 dilution (purified) + BxPC-3 cell lysate at 10 µg
Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 10 kDa
Observed band size: 10 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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Intracellular Flow Cytometry analysis of BxPC-3 (human Pancreas adenocarcinoma) cells labeling S100P with purified ab124743 at 1/1700 dilution (1ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
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Immunohistochemical staining of paraffin embedded human pancreatic adenocarcinoma with purified ab124743 at a working dilution of 1 in 300. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
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Immunofluorescence staining of BxPC-3 cells with purified ab124743 at a working dilution of 1 in 150, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 555 goat anti-rabbit, used at a dilution of 1 in 500. The cells were fixed in 4% PFA. The negative control is shown in bottom right hand panel - for the negative control, purified ab124743 was used at a dilution of 1/200 followed by an Alexa Fluor® 555 goat anti-mouse antibody at a dilution of 1/500.
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Anti-S100P antibody [EPR6142] (ab124743) at 1/1000 dilution (unpurified) + BxPC3 cell lysate at 10 µg
Secondary
Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 10 kDa
Observed band size: 10 kDa -
Unpurified ab124743, at 1/500 dilution, staining S100P in paraffin-embedded human pancreatic adenocarcinoma tissue by immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Unpurified ab124743, at 1/500 dilution, staining S100P in paraffin-embedded human placenta tissue by immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Unpurified ab124743, at 1/500 dilution, staining S100P in paraffin-embedded human spleen tissue by immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Unpurified ab124743 showing negative staining in normal liver tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Unpurified ab124743 showing positive staining in colonic adenocarcinoma tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Unpurified ab124743 showing negative staining in normal pancreas tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab124743 has been referenced in 3 publications.
- Zhang D et al. Promotion of the occurrence of endometrioid carcinoma by S100 calcium binding protein P. BMC Cancer 20:845 (2020). PubMed: 32883230
- Zhang X & Zhuang R Dione-thiophene conjugate inhibits proliferation and metastasis of nasopharyngeal carcinoma cells through calcium binding protein-P down-regulation. Eur J Med Chem 168:199-206 (2019). PubMed: 30822709
- Liu Y et al. S100P is associated with proliferation and migration in nasopharyngeal carcinoma. Oncol Lett 14:525-532 (2017). PubMed: 28693201