S12 antibody (ab43677)

Product Name 

S12 antibody

Product type 

Primary antibodies

Description 

Mouse polyclonal to S12

Immunogen 

Vector coding for a partial recombinant fusion protein: TVMFVTYCLI QLIKEKERRQ ALIDDPDPIP PPPPPAPVTA PTSTDPSS IQMDTTAAQP TETSKQKAE, corresponding to amino acids 471-537 of S12 from Medaka (Oryzias latipes).

Species Reactivity 

Reacts with Medaka (Oryzias latipes)
BLAST 'TVMFVTYCLI QLIKEKERRQ ALIDDPDPIP PPPPPAPVTA PTSTDPSS IQMDTTAAQP ' with or

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
WB: 1/1000.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Research areas 

Cell Biology >> Other Antibodies >> Other Antibodies

Relevance 

This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein.

A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein.

Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Raised in 

Mouse

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Whole antiserum

Storage buffer 

Preservative: None
Constituents: 50% Glycerol, Whole serum.

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

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