Anti-S12 antibody (ab43677)

Product name

Anti-S12 antibody

Description

Mouse polyclonal to S12

Tested applications

WBmore details

Cross reactivity

Reacts with Medaka (Oryzias latipes)

Immunogen

Vector coding for a partial recombinant fusion protein: TVMFVTYCLI QLIKEKERRQ ALIDDPDPIP PPPPPAPVTA PTSTDPSS IQMDTTAAQP TETSKQKAE, corresponding to amino acids 471-537 of S12 from Medaka (Oryzias latipes).

TVMFVTYCLI QLIKEKERRQ ALIDDPDPIP PPPPPAPVTA PTSTDPSS IQMDTTAAQP TETSKQKAE

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Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: 50% Glycerol, Whole serum.

Purity

Whole antiserum

Primary antibody notes

This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al.PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Clonality

Polyclonal

Isotype

IgG

Research areas

Cell Biology >> Other Antibodies >> Other Antibodies