Anti-S1P antibody (ab140592)
Key features and details
- Rabbit polyclonal to S1P
- Suitable for: WB
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
-
Product name
Anti-S1P antibody
See all S1P primary antibodies -
Description
Rabbit polyclonal to S1P -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Rabbit, Horse, Chicken, Human, Pig, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan -
Immunogen
-
Positive control
- WB: Mouse and rat liver tissue lysates.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab140592 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 118 kDa).
|
Notes |
---|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 118 kDa). |
Target
-
Relevance
S1P (also known as MBTPS1,SKI1), was first discovered as one of two enzymes that cleave the sterol regulatory binding proteins (SREBPs), in the case of S1P at “site-1,” a hydrophillic loop extending into the lumen of the ER. The MBTPS1 domain structure contains a signal sequence followed by a propeptide domain, a catalytic domain, a transmembrane domain and a cytoplasmic domain. -
Cellular localization
Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein. -
Database links
- Entrez Gene: 100689417 Chinese hamster
- Entrez Gene: 8720 Human
- Entrez Gene: 56453 Mouse
- Entrez Gene: 89842 Rat
- Omim: 603355 Human
- SwissProt: Q9Z2A8 Chinese hamster
- SwissProt: Q14703 Human
- SwissProt: Q9WTZ2 Mouse
see all -
Alternative names
- kexin isozyme 1 antibody
- KIAA0091 antibody
- MBTPS1 antibody
see all
Images
-
All lanes : Anti-S1P antibody (ab140592) at 1 µg/ml
Lane 1 : Mouse liver tissue lysate
Lane 2 : Rat liver tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 118 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Additional bands at: 62 kDa (possible non-specific binding)
Exposure time: 90 secondsThe band observed at 100 kDa could potentially be a cleaved form of S1P due to the presence of a 17 amino acid signal peptide and a 169 amino acid pro-peptide.
This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab140592 overnight at 4°C.
Datasheets and documents
-
SDS download
-
Datasheet download
References (6)
ab140592 has been referenced in 6 publications.
- Jung Y et al. Dysregulation of sphingosine-1-phosphate (S1P) and S1P receptor 1 signaling in the 5xFAD mouse model of Alzheimer's disease. Brain Res 1799:148171 (2023). PubMed: 36410428
- Shibuya K et al. AAA-ATPase valosin-containing protein binds the transcription factor SREBP1 and promotes its proteolytic activation by rhomboid protease RHBDL4. J Biol Chem 298:101936 (2022). PubMed: 35430252
- Li Y & Wu S Curcumin inhibits the proteolytic process of SREBP-2 by first inhibiting the expression of S1P rather than directly inhibiting SREBP-2 expression. Food Sci Nutr 9:209-216 (2021). PubMed: 33473285
- Molstad DHH et al. Hdac3 regulates bone modeling by suppressing osteoclast responsiveness to RANKL. J Biol Chem 295:17713-17723 (2020). PubMed: 33454009
- Guo C et al. Cholesterol Homeostatic Regulator SCAP-SREBP2 Integrates NLRP3 Inflammasome Activation and Cholesterol Biosynthetic Signaling in Macrophages. Immunity 49:842-856.e7 (2018). PubMed: 30366764
- Kim JY et al. ER Stress Drives Lipogenesis and Steatohepatitis via Caspase-2 Activation of S1P. Cell 175:133-145.e15 (2018). PubMed: 30220454