![Western blot - P70 S6 Kinase antibody [E343] (ab32529) image](#ab32529wb.jpg)
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Product name
Anti-S6K antibody [E343]
See all S6K products (39) ...
Description
Rabbit monoclonal [E343] to S6K
Specificity
This antibody detects both alpha I and alpha II isoforms.
Tested applications
WB, IHC-P, Flow Cyt, IPmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
A synthetic peptide corresponding to residues in N-terminus of human p70 S6 kinase.
Positive control
293T cell lysate and human breast adenocarcinoma tissue. Mouse and Rat heart lysates.
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Monoclonal
Clone number
E343
Isotype
IgG
Research areas
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> S6K Family
Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Ribosome
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> DNA Damage Recognition
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Rb
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Western blot - P70 S6 Kinase antibody [E343] (ab32529)
![Western blot - P70 S6 Kinase antibody [E343] (ab32529) image](/ps/datasheet/Images/32/ab32529/ab32529wb.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab32529 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/5000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 59 kDa).For Rat and Mouse samples 1/500 dilution has only been tried. We have not tested if similarly to Human samples a lot higher dilutions can be used.
IHC-P
IHC-P: 1/100 - 1/250.IHC-P: 1/100 - 1/250.
Flow Cyt
Flow Cyt: 1/20.Flow Cyt: 1/20.
IP
IP: 1/80.IP: 1/80.
Application notes
Is unsuitable for or ICC.
Target
Function
Acts to integrate nutrient and growth factor signals in regulation of protein synthesis, cell proliferation, cell growth, cell cycle progression and cell survival. Downstream effector of the mTOR signaling pathway. Phosphorylates specifically ribosomal protein S6 in response to insulin or several classes of mitogens. During translation initiation, the inactive form associatess with the eIF-3 complex under conditions of nutrient depletion. Mitogenic stimulation leads to phosphorylation and dissociation from the eIF-3 complex and the free activated form can phosphorylate other translational targets including EIF4B. Promotes protein synthesis by phosphorylating PDCD4 at 'Ser-67' and targeting it for degradation. Phosphorylates RICTOR leading to regulation of mammalian target of rapamycin complex 2 (mTORC2) signaling; probably phosphorylates RICTOR at 'Thr-1135'. Phosphorylates IRS1 at multiple serine residues coupled with insulin resistance; probably phosphorylates IRS1 at 'Ser-270'. Required for TNF-alpha induced IRS-1 degradation. Phosphorylates EEF2K in response to IGF1 and inhibits EEF2K activity. Phosphorylates BAD at 'Ser-99' in response to IGF1 leading to BAD inactivation and inhibition of BAD-induced apoptosis. Phosphorylates mitochondrial RMP leading to dissociation of a RMP:PPP1CC complex; probably phosphorylates RMP at 'Ser-99'. The free mitochondrial PPP1CC can dephosphorylate RPS6KB1 at Thr-412 which is proposed to be a negative feed back mechanism for the RPS6KB1 antiapoptotic function. Phosphorylates GSK3B at 'Ser-9' under conditions leading to loss of the TSC1-TSC2 complex. Phosphorylates POLDIP3.
Tissue specificity
Widely expressed.
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 protein kinase domain.
Domain
The autoinhibitory domain is believed to block phosphorylation within the AGC-kinase C-terminal domain and the activation loop.
The TOS (TOR signaling) motif is essential for activation by mTORC1.
Post-translational
modifications
Phosphorylation at Thr-412 is regulated by mTORC1. The phosphorylation at this site is maintained by an agonist-dependent autophosphorylation mechanism.
Cellular localization
Cytoplasm; Nucleus. Cytoplasm and Cell junction > synapse > synaptosome. Mitochondrion outer membrane.
Target information above from: UniProt accessionP23443
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 70 kDa ribosomal protein S6 kinase 1 antibody
- KS6B1_HUMAN antibody
- p70 alpha antibody
see all
Database links
- Entrez Gene: 6198 Human
- Entrez Gene: 72508 Mouse
- Entrez Gene: 83840 Rat
- Omim: 608938 Human
- SwissProt: P23443 Human
- SwissProt: Q8BSK8 Mouse
- SwissProt: P67999 Rat
- Unigene: 463642 Human
see all
Anti-S6K antibody [E343] images:
Western blot - P70 S6 Kinase antibody [E343] (ab32529)
Anti-S6K antibody [E343] (ab32529) at 1/10000 dilution + 293T cell lysate
Predicted band size : 59 kDa
Observed band size : 70 kDa (why is the actual band size different from the predicted?)
References for Anti-S6K antibody [E343] (ab32529)
ab32529 has not yet been referenced specifically in any publications.
Publishing research using ab32529? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"