Overview
- Product nameAnti-S6K1 antibodySee all S6K1 primary antibodies ...
- DescriptionRabbit polyclonal to S6K1
- SpecificityReacts strongly with 70 kd of p70 S6K, but only weakly with the 80 kDa S6K.
- Tested applicationsWB, ELISA, IP more details
- Species reactivityReacts with: Mouse, Rat, Rabbit, Cow, Human, Pig
- Immunogen
S6K c-terminal peptide conjugated to KLH and BSA.
- EpitopeThe antigen is based on the C-terminal sequence of human p70 S6K (C-ISKRPEHLRMNL).
- Positive controlUse Mouse brain extract.
Properties
- FormLiquid
- Storage instructionsStore at +4°C. Do not freeze.
- Storage bufferPBS with 0.02% sodium azide
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Concentration information loading... - PurityImmunogen affinity purified
- Purification notesImmunoaffinity chromatography with S6K C-terminal peptide on agarose.
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab9366 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: 1/250. |
| ELISA | ELISA: 1/500. |
| IP | IP: Use at 3 µg/mg of lysate. |
Target
- FunctionActs to integrate nutrient and growth factor signals in regulation of protein synthesis, cell proliferation, cell growth, cell cycle progression and cell survival. Downstream effector of the mTOR signaling pathway. Phosphorylates specifically ribosomal protein S6 in response to insulin or several classes of mitogens. During translation initiation, the inactive form associatess with the eIF-3 complex under conditions of nutrient depletion. Mitogenic stimulation leads to phosphorylation and dissociation from the eIF-3 complex and the free activated form can phosphorylate other translational targets including EIF4B. Promotes protein synthesis by phosphorylating PDCD4 at 'Ser-67' and targeting it for degradation. Phosphorylates RICTOR leading to regulation of mammalian target of rapamycin complex 2 (mTORC2) signaling; probably phosphorylates RICTOR at 'Thr-1135'. Phosphorylates IRS1 at multiple serine residues coupled with insulin resistance; probably phosphorylates IRS1 at 'Ser-270'. Required for TNF-alpha induced IRS-1 degradation. Phosphorylates EEF2K in response to IGF1 and inhibits EEF2K activity. Phosphorylates BAD at 'Ser-99' in response to IGF1 leading to BAD inactivation and inhibition of BAD-induced apoptosis. Phosphorylates mitochondrial RMP leading to dissociation of a RMP:PPP1CC complex; probably phosphorylates RMP at 'Ser-99'. The free mitochondrial PPP1CC can dephosphorylate RPS6KB1 at Thr-412 which is proposed to be a negative feed back mechanism for the RPS6KB1 antiapoptotic function. Phosphorylates GSK3B at 'Ser-9' under conditions leading to loss of the TSC1-TSC2 complex. Phosphorylates POLDIP3.
- Tissue specificityWidely expressed.
- Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 protein kinase domain. - DomainThe autoinhibitory domain is believed to block phosphorylation within the AGC-kinase C-terminal domain and the activation loop.
The TOS (TOR signaling) motif is essential for activation by mTORC1. - Post-translational
modificationsPhosphorylation at Thr-412 is regulated by mTORC1. The phosphorylation at this site is maintained by an agonist-dependent autophosphorylation mechanism. - Cellular localizationCytoplasm; Nucleus. Cytoplasm and Cell junction > synapse > synaptosome. Mitochondrion outer membrane.
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Database links
- Entrez Gene: 6198 Human
- Entrez Gene: 72508 Mouse
- Entrez Gene: 83840 Rat
- Omim: 608938 Human
- SwissProt: P23443 Human
- SwissProt: Q8BSK8 Mouse
- SwissProt: P67999 Rat
- Unigene: 463642 Human
- Unigene: 394280 Mouse
- Unigene: 446624 Mouse
- Unigene: 479484 Mouse
- Unigene: 4042 Rat
see all
Target information above from: UniProt accession
P23443
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- 70 kDa ribosomal protein S6 kinase 1 antibodyKS6B1_HUMAN antibodyp70 alpha antibody
- P70 beta 1 antibodyP70 beta 1 antibodyp70 ribosomal S6 kinase alpha antibodyp70 ribosomal S6 kinase beta 1 antibodyp70 ribosomal S6 kinase beta 1 antibodyp70 S6 kinase alpha antibodyP70 S6 Kinase antibodyp70 S6 kinase, alpha 1 antibodyp70 S6 kinase, alpha 2 antibodyp70 S6K antibodyp70 S6K-alpha antibodyp70 S6KA antibodyp70(S6K) alpha antibodyp70(S6K)-alpha antibodyp70-alpha antibodyp70-S6K 1 antibodyp70-S6K antibodyP70S6K antibodyP70S6K1 antibodyp70S6Kb antibodyp70S6Kb antibodyPS6K antibodyRibosomal protein S6 kinase 70kDa polypeptide 1 antibodyRibosomal protein S6 kinase 70kDa polypeptide 2 antibodyRibosomal protein S6 kinase 70kDa polypeptide 2 antibodyRibosomal protein S6 kinase beta 1 antibodyRibosomal protein S6 kinase beta-1 antibodyRibosomal protein S6 kinase I antibodyRPS6KB1 antibodyRPS6KB2 antibodyRPS6KB2 antibodyS6K antibodyS6K-beta-1 antibodyS6K1 antibodySerine/threonine kinase 14 alpha antibodySerine/threonine-protein kinase 14A antibodySTK14A antibody
see all
Anti-S6K1 antibody images
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Western blotOne-step immunoblotting with cross-antibodies of the immunoprecipitated kinases
Immunoprecipitation with anti-p90 RSK1 (Lane A and C)
Immunoprecipitation with anti-p70 S6K (Lane B and D)
Immunoblotting with anti-p70 S6K HRPconjugates (Lane A and B)
Immunoblotting with anti-RSK1 HRP conjugates (Lane C and D) -
Western blotOne-step immunoblotting of crude mouse brain and muscle samples with anti-S6K HRP conjugates (ECL system).
Lane A: mouse Muscle
Lane B: mouse Brain
References for Anti-S6K1 antibody (ab9366)
This product has been referenced in:
- Gao Z et al. Inactivation of NF-{kappa}B p50 Leads to Insulin Sensitization in Liver through Post-translational Inhibition of p70S6K. J Biol Chem 284:18368-76 (2009). WB ; Mouse . Read more (PubMed: 19433583) »
- Zhang J et al. S6K directly phosphorylates IRS-1 on Ser-270 to promote insulin resistance in response to TNF-(alpha) signaling through IKK2. J Biol Chem 283:35375-82 (2008). WB ; Mouse . Read more (PubMed: 18952604) »
