![Western blot - P70 S6 Kinase (phospho T421 + S424) antibody [E135] (ab32525) image](#ab32525wb.gif)
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Product name
Anti-S6K (phospho T421 + S424) antibody [E135]
See all S6K products (39) ...
Description
Rabbit monoclonal [E135] to S6K (phospho T421 + S424)
Specificity
The antibody only detects p70-S6K phosphorylated on Threonine 421 and Serine 424.
Tested applications
WB, IPmore details
Cross reactivity
Reacts with
Human
Does not react with
Mouse, Rat
Immunogen
A synthetic phospho-peptide corresponding to residues surrounding Thr421 and Ser424 of human p70 S6 Kinase alpha.
Positive control
293T cell lysate.
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Monoclonal
Clone number
E135
Isotype
IgG
Research areas
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> S6K Family
Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Ribosome
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> DNA Damage Recognition
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Rb
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Western blot - P70 S6 Kinase (phospho T421 + S424) antibody [E135] (ab32525)
![Western blot - P70 S6 Kinase (phospho T421 + S424) antibody [E135] (ab32525) image](/ps/datasheet/Images/32/ab32525/ab32525wb.gif)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab32525 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
IP
Application notes
IP: 1/20.
WB: 1/2500. Detects a band of approximately 70 kDa (predicted molecular weight: 59 kDa).
Is unsuitable for FACS, ICC or IHC.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Acts to integrate nutrient and growth factor signals in regulation of protein synthesis, cell proliferation, cell growth, cell cycle progression and cell survival. Downstream effector of the mTOR signaling pathway. Phosphorylates specifically ribosomal protein S6 in response to insulin or several classes of mitogens. During translation initiation, the inactive form associatess with the eIF-3 complex under conditions of nutrient depletion. Mitogenic stimulation leads to phosphorylation and dissociation from the eIF-3 complex and the free activated form can phosphorylate other translational targets including EIF4B. Promotes protein synthesis by phosphorylating PDCD4 at 'Ser-67' and targeting it for degradation. Phosphorylates RICTOR leading to regulation of mammalian target of rapamycin complex 2 (mTORC2) signaling; probably phosphorylates RICTOR at 'Thr-1135'. Phosphorylates IRS1 at multiple serine residues coupled with insulin resistance; probably phosphorylates IRS1 at 'Ser-270'. Required for TNF-alpha induced IRS-1 degradation. Phosphorylates EEF2K in response to IGF1 and inhibits EEF2K activity. Phosphorylates BAD at 'Ser-99' in response to IGF1 leading to BAD inactivation and inhibition of BAD-induced apoptosis. Phosphorylates mitochondrial RMP leading to dissociation of a RMP:PPP1CC complex; probably phosphorylates RMP at 'Ser-99'. The free mitochondrial PPP1CC can dephosphorylate RPS6KB1 at Thr-412 which is proposed to be a negative feed back mechanism for the RPS6KB1 antiapoptotic function. Phosphorylates GSK3B at 'Ser-9' under conditions leading to loss of the TSC1-TSC2 complex. Phosphorylates POLDIP3.
Tissue specificity
Widely expressed.
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 protein kinase domain.
Domain
The autoinhibitory domain is believed to block phosphorylation within the AGC-kinase C-terminal domain and the activation loop.
The TOS (TOR signaling) motif is essential for activation by mTORC1.
Post-translational
modifications
Phosphorylation at Thr-412 is regulated by mTORC1. The phosphorylation at this site is maintained by an agonist-dependent autophosphorylation mechanism.
Cellular localization
Cytoplasm; Nucleus. Cytoplasm and Cell junction > synapse > synaptosome. Mitochondrion outer membrane.
Target information above from: UniProt accessionP23443
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 70 kDa ribosomal protein S6 kinase 1 antibody
- KS6B1_HUMAN antibody
- p70 alpha antibody
see all
Database links
- Entrez Gene: 6198 Human
- Omim: 608938 Human
- SwissProt: P23443 Human
- Unigene: 463642 Human
Anti-S6K (phospho T421 + S424) antibody [E135] images:
Western blot - P70 S6 Kinase (phospho T421 + S424) antibody [E135] (ab32525)
All lanes : Anti-S6K (phospho T421 + S424) antibody [E135] (ab32525) at 1/2500 dilution
Lane 1 : 293T cell lysate, untreated
Lane 2 : 293T cell lysate, treated with EGF
Predicted band size : 59 kDa
Observed band size : 70 kDa (why is the actual band size different from the predicted?)
References for Anti-S6K (phospho T421 + S424) antibody [E135] (ab32525)
ab32525 has not yet been referenced specifically in any publications.
Publishing research using ab32525? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"