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Products:Cell Biology >> Cell Cycle >> Cell Division >> Chromatid Cohesion
Overview
Product name
Anti-SA1 antibody - ChIP Grade
See all SA1 products (3) ...
Description
Goat polyclonal to SA1 - ChIP Grade
Tested applications
WB, ICC, ChIP, ChIP/Chipmore details
Cross reactivity
Reacts with
Human, Xenopus laevis
Immunogen
Synthetic peptide (Human) - 35 amino acids which represented a portion of SA 1 encoded in part by exons 35 and 36.
Epitope
The epitope recognized by ab4457 maps to a region between residues 1150 and 1200 of human Stromal Antigen 1 using the numbering given in entry NP_005853.2 (GeneID 10274).
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
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Western blot - SA1 antibody - ChIP Grade (ab4457)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab4457 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
ICC
ChIP
ChIP/Chip
Application notes
ChIP: Use at an assay dependent dilution (PMID 18550811).
ChIP/Chip: Use at an assay dependent dilution (PMID 18550811).
ICC: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Detects a band of approximately 144 kDa (predicted molecular weight: 144 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis.
Sequence similarities
Belongs to the SCC3 family.
Contains 1 SCD (stromalin conservative) domain.
Post-translational
modifications
Phosphorylated by PLK. The large dissociation of cohesin from chromosome arms during prophase is partly due to its phosphorylation.
Cellular localization
Nucleus. Chromosome. Chromosome > centromere. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of cohesin is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation.
Target information above from: UniProt accessionQ8WVM7
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Cohesin subunit SA 1 antibody
- Cohesin subunit SA-1 antibody
- Cohesin Subunit SA1 antibody
see all
Database links
- Entrez Gene: 10274 Human
- Entrez Gene: 399163 Xenopus laevis
- Omim: 604358 Human
- SwissProt: Q8WVM7 Human
- SwissProt: Q9DGN1 Xenopus laevis
- Unigene: 412586 Human
- Unigene: 3441 Xenopus laevis
Anti-SA1 antibody - ChIP Grade images:
Western blot - SA1 antibody - ChIP Grade (ab4457)
Predicted band size : 144 kDa
Nuclear Extract from HeLa cells (~5 x 105 cells).
ab4457 at 0.5 µg/ml.
Detection: ECL with 15 second exposure.
References for Anti-SA1 antibody - ChIP Grade (ab4457)
This product has been referenced in:
- Schmidt Det al. A CTCF-independent role for cohesin in tissue-specific transcription. Genome Res 20:578-88 (2010). ChIP; Human.Read more (PubMed: 20219941) »
- Rubio EDet al. CTCF physically links cohesin to chromatin. Proc Natl Acad Sci U S A 105:8309-14 (2008). ChIP, ChIP/Chip; Human.Read more (PubMed: 18550811) »
See all 2 publications for this product
Publishing research using ab4457? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"