Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Cell Biology >> Cell Cycle >> Cell Division >> Chromatid Cohesion
Overview
Product name
Anti-SA1 antibody
See all SA1 products (3) ...
Description
Goat polyclonal to SA1
Tested applications
WB, ICC, ICC/IF, IPmore details
Cross reactivity
Reacts with
Human, Xenopus laevis
Immunogen
Synthetic peptide (Human) - 36 amino acids which represent a portion of SA 1 encoded in part by exons 5 and 6.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Cell cycle >> Chromosome Structure >> Chromatid Cohesion
Cell Biology >> Cell Cycle >> Cell Division >> Chromatid Cohesion
Applications
Show applications key
Our Abpromise guarantee covers the use of ab4455 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
ICC
IP
Application notes
ICC: 1/500 - 1/2000.
ICC/IF: 1/500.
IP: Use at an assay dependent dilution. (PMID 20124417)
WB: 1/1000 - 1/10000. Detects a band of approximately 144 kDa (predicted molecular weight: 144 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis.
Sequence similarities
Belongs to the SCC3 family.
Contains 1 SCD (stromalin conservative) domain.
Post-translational
modifications
Phosphorylated by PLK. The large dissociation of cohesin from chromosome arms during prophase is partly due to its phosphorylation.
Cellular localization
Nucleus. Chromosome. Chromosome > centromere. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of cohesin is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation.
Target information above from: UniProt accessionQ8WVM7
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Cohesin subunit SA 1 antibody
- Cohesin subunit SA-1 antibody
- Cohesin Subunit SA1 antibody
see all
Database links
- Entrez Gene: 10274 Human
- Entrez Gene: 399163 Xenopus laevis
- Omim: 604358 Human
- SwissProt: Q8WVM7 Human
- SwissProt: Q9DGN1 Xenopus laevis
- Unigene: 412586 Human
- Unigene: 3441 Xenopus laevis
Anti-SA1 antibody images:
Western blot - SA1 antibody (ab4455)
Predicted band size : 144 kDa
Nuclear Extract from HeLa cells (~5 x 105 cells).
ab4455 at 0.5 µg/ml.
Detection: ECL with 15 second exposure.
Immunocytochemistry/ Immunofluorescence - SA1 antibody (ab4455)
Goat polyclonal to SA1 antibody (ab4455) at a 1/500 dilution detecting Stromal Antigen 1 in assynchronous HeLa cells. Cells were fixed with 4% paraformaldehyde and counterstained with DAPI. Secondary antibody:Donkey anti-goat IgG conjugated to Cy3. For further information please refer to abreview.
This image is courtesy of an Abreview submitted by Dr Kirk McManus
References for Anti-SA1 antibody (ab4455)
This product has been referenced in:
- Xiao Tet al. Specific Sites in the C Terminus of CTCF Interact with the SA2 Subunit of the Cohesin Complex and Are Required for Cohesin-Dependent Insulation Activity. Mol Cell Biol 31:2174-83 (2011). WB; Human.Read more (PubMed: 21444719) »
- Leman ARet al. Human Timeless and Tipin stabilize replication forks and facilitate sister-chromatid cohesion. J Cell Sci 123:660-70 (2010). WB, IP; Human.Read more (PubMed: 20124417) »
See all 2 publications for this product
Publishing research using ab4455? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"