Anti-SA2 antibody [3C6] (ab117725)

Overview

• Product nameAnti-SA2 antibody [3C6]See all SA2 primary antibodies ...
• Description
  Mouse monoclonal [3C6] to SA2
• Tested applicationsWB, IHC-P, ICC/IF more details
• Species reactivity
  Reacts with: Mouse, Human
  
• Immunogen

  Recombinant Human SA2 Protein (Q8N3U4).

• Positive controlHuman Breast tissue, Hela nuclear and NIH/3T3 cells extracts and HeLa cells.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
• Storage bufferpH: 7.20
  Constituent: 99% PBS
• Concentration information loading...
• PurityProtein A purified
• Clonality Monoclonal
• Clone number3C6
• IsotypeIgG1
• Light chain typekappa
• Research Areas
  • Epigenetics and Nuclear Signaling
  • Cell cycle
  • Chromosome Structure
  • Chromatid Cohesion

  • Cell Biology
  • Cell Cycle
  • Cell Division
  • Chromatid Cohesion

Applications

Target

• FunctionComponent of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis.
• Sequence similaritiesBelongs to the SCC3 family.
  Contains 1 SCD (stromalin conservative) domain.
• Post-translational
  modificationsPhosphorylated by PLK. The large dissociation of cohesin from chromosome arms during prophase is partly due to its phosphorylation.
• Cellular localizationNucleus. Chromosome. Chromosome > centromere. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of cohesin is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. In germ cells, cohesin complex dissociates from chromatin at prophase I, and may be replaced by a meiosis-specific cohesin complex.

Target information above from: UniProt accession Q8N3U4 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 10735 Human
  • Entrez Gene: 10735 Human
  • Entrez Gene: 20843 Mouse
  • Omim: 300826 Human
  • SwissProt: Q8N3U4 Human
  • SwissProt: Q8N3U4 Human
  • SwissProt: O35638 Mouse
  • Unigene: 496710 Human

  • Unigene: 624663 Human
  • Unigene: 290422 Mouse

  see all

• Alternative names
  bA517O1.1 antibodyCohesin Subunit SA 2 antibodyCohesin subunit SA-2 antibody

  DKFZp686P168 antibodyDKFZp781H1753 antibodyFLJ25871 antibodySA 2 antibodySA-2 antibodySA2 antibodySCC3 homolog 2 antibodySCC3B antibodySTAG 2 antibodystag2 antibodySTAG2_HUMAN antibodyStromal antigen 2 antibody

  see all

Anti-SA2 antibody [3C6] images

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SA2 antibody [3C6] (ab117725)
  Immunohistochemistry Image of Human Breast: Formalin-Fixed, Paraffin-Embedded stained with anti SA2 antibody at 5 µg/ml(ab117725).
• 

  Western blot - Anti-SA2 antibody [3C6] (ab117725)
  Anti-SA2 antibody [3C6] (ab117725) + Hela nuclear extracts
  
  Predicted band size : 141 kDa
  
• 

  Western blot - Anti-SA2 antibody [3C6] (ab117725)
  Anti-SA2 antibody [3C6] (ab117725) + NIH/3T3 protein cell extracts
  
  Predicted band size : 141 kDa
  
• 

  Immunocytochemistry/ Immunofluorescence - Anti-SA2 antibody [3C6] (ab117725)
  Immunofluorescence of monoclonal antibody to anti SA2 antibody (ab117725) at 10 µg/ml on HeLa cell.