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Products:Cardiovascular >> Blood >> Acute Phase Reactants
Overview
Product name
Anti-SAA4 antibody
See all SAA4 products (4) ...
Description
Mouse polyclonal to SAA4
Tested applications
IHC-P, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Full length protein, corresponding to amino acids 1-130 of Human SAA4
Positive control
Human stomach tissue lysate; SAA4 transfected 293T cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 1X PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab67358 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use a concentration of 1 µg/mlPerform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB: 1/500 - 1/1000.Detects a band of approximately 17 kDa (predicted molecular weight: 15 kDa).
Target
Relevance
SAA4 is a constitutively expressed protein belonging to the SAA family. It is a major acute phase reactant and an apolipoprotein of the HDL complex. The serum amyloid A (SAA) superfamily comprises a number of differentially expressed genes with a high degree of homology in mammalian species. SAA4, an apolipoprotein constitutively expressed only in humans and mice, is associated almost entirely with lipoproteins of the high density range. Its physiological function is unknown and its serum concentration has no relationship with those of other major apolipoproteins. The presence of SAA4 mRNA and protein in macrophage derived foam cells of coronary and carotid arteries suggested a specific role of human SAA4 during inflammation including atherosclerosis.
Cellular localization
Secreted
Alternative names
- C SAA antibody
- Constitutively expressed serum amyloid A protein antibody
- CSAA antibody
see all
Database links
- Entrez Gene: 6291 Human
- Omim: 104752 Human
- SwissProt: P35542 Human
Anti-SAA4 antibody images:
Western blot - SAA4 antibody (ab67358)
Anti-SAA4 antibody (ab67358) at 1/500 dilution + Human stomach tissue lysate at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 15 kDa
Observed band size : 17 kDa (why is the actual band size different from the predicted?)
Additional bands at : 13 kDa. We are unsure as to the identity of these extra bands.
Western blot - SAA4 antibody (ab67358)
All lanes : Anti-SAA4 antibody (ab67358) at 1/500 dilution
Lane 1 : SAA4 transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 15 kDa
Observed band size : 13 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SAA4 antibody (ab67358)
IHC image of ab67358 staining in human normal stomach formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab67358, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
References for Anti-SAA4 antibody (ab67358)
ab67358 has not yet been referenced specifically in any publications.
Publishing research using ab67358? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"