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Analysis of SAA4 Recombinant Protein (ab40603) @ 1µg using a 4-20% SDS gradient gel. Coomassie blue staining. A band is seen at about 17 kDa.
All lanes : Mouse anti-SAA4 polyclonal at 10 µg/ml Image courtesy of an anonymous Abreview.
Lane 1 : Whole tissue lysate prepared from whole human acute phase plasma, heated to 95°C in 10-fold excess of reducing SDS sample buffer for 10 minutes. at 14 µg/ml
Lane 2 : Whole tissue lysate prepared from whole human acute phase plasma, heated to 95°C in 10-fold excess of reducing SDS sample buffer for 10 minutes. at 14 µg/ml
Lane 3 : Recombinant A-SAA at 14 µg/ml
Lane 4 :
Secondary
Goat anti-mouse IgG-HRP at 1/10000 dilution
developed using the ECL technique
Predicted band size : 16 kDa
Observed band size : 16 kDa
Exposure time : 2 minutes
1
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