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Products:Cardiovascular >> Blood >> Acute Phase Reactants
Overview
Product name
SAA4 protein (His tag)
Protein description
Recombinant fragment, corresponding to amino acids 19-130 of Human SAA4 (with His-Tag at the N-terminus.)
Expression host
E. coli
Properties
Purity
>95% by SDS-PAGE
Form
Liquid
Storage instructions
Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.002% Sodium Azide
Constituents: 0.1% Triton-X-100, 10mM Tris, 10mM DTT, pH 8.0
Concentration
Concentration information loading...
Applications
Show applications key
Our Abpromise guarantee covers the use of ab40603 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
MS: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 16 kDa.
SDS-PAGE: Use at an assay dependent dilution.
Protein info
Relevance
SAA4 is a constitutively expressed protein belonging to the SAA family. It is a major acute phase reactant and an apolipoprotein of the HDL complex. The serum amyloid A (SAA) superfamily comprises a number of differentially expressed genes with a high degree of homology in mammalian species. SAA4, an apolipoprotein constitutively expressed only in humans and mice, is associated almost entirely with lipoproteins of the high density range. Its physiological function is unknown and its serum concentration has no relationship with those of other major apolipoproteins. The presence of SAA4 mRNA and protein in macrophage derived foam cells of coronary and carotid arteries suggested a specific role of human SAA4 during inflammation including atherosclerosis.
Cellular localization
Secreted
SAA4 protein (His tag) images:
SDS-PAGE - SAA4 peptide (19-130) (ab40603)
Analysis of SAA4 Recombinant Protein (ab40603) @ 1µg using a 4-20% SDS gradient gel. Coomassie blue staining. A band is seen at about 17 kDa.
Western blot - SAA4 protein (His tag) (ab40603)
All lanes : Mouse anti-SAA4 polyclonal at 10 µg/ml
Lane 1 : Whole tissue lysate prepared from whole human acute phase plasma, heated to 95°C in 10-fold excess of reducing SDS sample buffer for 10 minutes. at 14 µg/ml
Lane 2 : Whole tissue lysate prepared from whole human acute phase plasma, heated to 95°C in 10-fold excess of reducing SDS sample buffer for 10 minutes. at 14 µg/ml
Lane 3 : Recombinant A-SAA at 14 µg/ml
Lane 4 : SAA4 protein (His tag) (ab40603) at 10 µg
Secondary
Goat anti-mouse IgG-HRP at 1/10000 dilution
developed using the ECL technique
Predicted band size : 16 kDa
Observed band size : 16 kDa
Exposure time : 2 minutes
Image courtesy of an anonymous Abreview.
References for SAA4 protein (His tag) (ab40603)
ab40603 has not yet been referenced specifically in any publications.
Publishing research using ab40603? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"