Anti-SAE1 antibody (ab38434)

Overview

• Product nameAnti-SAE1 antibodySee all SAE1 primary antibodies ...
• Description
  Rabbit polyclonal to SAE1
• Tested applicationsELISA, WB, IHC-P more details
• Species reactivity
  Reacts with: Mouse, Human
  
• Immunogen

  Synthetic peptide conjugated to KLH, derived from within residues 10-30 at the C terminal of the last 50 amino acids of AOS1 (Human).

• Positive controlMouse heart tissue lysate (WB). Human breast carcinoma tissue.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.09% Sodium Azide
  Constituents: PBS
• Concentration information loading...
• PurityProtein G purified
• Purification notesThis antibody is eluted out with both high and low pH buffers, neutralized immediately after elution then followed by dialysis against PBS.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Cell Biology
  • Proteolysis / Ubiquitin
  • Proteasome / Ubiquitin
  • Sumo

  • Cell Biology
  • Proteolysis / Ubiquitin
  • Proteasome / Ubiquitin
  • Ub-like Proteins

Applications

Target

• FunctionThe heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
• Tissue specificityExpression level increases during S phase and drops in G2 phase (at protein level).
• PathwayProtein modification; protein sumoylation.
• Sequence similaritiesBelongs to the ubiquitin-activating E1 family.
• Post-translational
  modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
• Cellular localizationNucleus.

Target information above from: UniProt accession Q9UBE0 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 10055 Human
  • Entrez Gene: 56459 Mouse
  • Omim: 613294 Human
  • SwissProt: Q9UBE0 Human
  • SwissProt: Q9R1T2 Mouse
  • Unigene: 515500 Human
  • Unigene: 258530 Mouse

• Alternative names
  Activator of SUMO1 antibodyAOS 1 antibodyAOS1 antibody

  FLJ3091 antibodyHSPC 140 antibodyHSPC140 antibodySAE 1 antibodySae1 antibodySAE1_HUMAN antibodySentrin/SUMO activating protein AOS 1 antibodySentrin/SUMO activating protein AOS1 antibodySUA 1 antibodySUA1 antibodySUMO 1 activating enzyme E1 N subunit antibodySUMO 1 activating enzyme subunit 1 antibodySUMO-activating enzyme subunit 1 antibodySUMO1 activating enzyme E1 N subunit antibodySUMO1 activating enzyme subunit 1 antibodyUbiquitin like 1 activating enzyme E1A antibodyUbiquitin like protein SUMO 1 activating enzyme antibodyUbiquitin like protein SUMO1 activating enzyme antibodyUbiquitin-like 1-activating enzyme E1A antibodyUBL E1A antibodyUBLE 1A antibodyUBLE1A antibody

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Anti-SAE1 antibody images

• 

  Western blot - Aos1 + Uba2 antibody (ab38434)
  Anti-SAE1 antibody (ab38434) + Mouse heart tissue lysate
  
  Predicted band size : 38 kDa
  Additional bands at : 59 kDa,70 kDa,75 kDa. We are unsure as to the identity of these extra bands.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Aos1 + Uba2 antibody (ab38434)
  Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with ab38434, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining