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Products:Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Sumo
Overview
Product name
Anti-SAE1 antibody
See all SAE1 products (9) ...
Description
Rabbit polyclonal to SAE1
Tested applications
ELISA, WB, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide conjugated to KLH, derived from within residues 10-30 at the C terminal of the last 50 amino acids of AOS1 (Human).
Positive control
Mouse heart tissue lysate (WB). Human breast carcinoma tissue.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
This antibody is eluted out with both high and low pH buffers, neutralized immediately after elution then followed by dialysis against PBS.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ub-like Proteins
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Sumo
Applications
Show applications key
Our Abpromise guarantee covers the use of ab38434 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/1000ELISA: 1/1000
WB: 1/100 - 1/500.Predicted molecular weight: 38 kDa.
IHC-P: 1/50 - 1/100.
Target
Function
The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
Tissue specificity
Expression level increases during S phase and drops in G2 phase (at protein level).
Pathway
Protein modification; protein sumoylation.
Sequence similarities
Belongs to the ubiquitin-activating E1 family.
Post-translational
modifications
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ9UBE0
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AOS 1 antibody
- AOS1 antibody
- FLJ3091 antibody
see all
Database links
- Entrez Gene: 10055 Human
- Entrez Gene: 56459 Mouse
- Omim: 613294 Human
- SwissProt: O95717 Human
- SwissProt: Q9UBE0 Human
- SwissProt: Q9R1T2 Mouse
- Unigene: 515500 Human
- Unigene: 258530 Mouse
Anti-SAE1 antibody images:
Western blot - Aos1 + Uba2 antibody (ab38434)
Anti-SAE1 antibody (ab38434) + Mouse heart tissue lysate
Predicted band size : 38 kDa
Additional bands at : 59 kDa,70 kDa,75 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Aos1 + Uba2 antibody (ab38434)
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with ab38434, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining
References for Anti-SAE1 antibody (ab38434)
ab38434 has not yet been referenced specifically in any publications.
Publishing research using ab38434? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"