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Rabbit polyclonal to SAE1
Synthetic peptide conjugated to KLH, derived from within residues 10-30 at the C terminal of the last 50 amino acids of AOS1 (Human).
Mouse heart tissue lysate (WB).
Human breast carcinoma tissue.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Preservative: 0.09% Sodium Azide
Concentration information loading...
Protein G purified
This antibody is eluted out with both high and low pH buffers, neutralized immediately after elution then followed by dialysis against PBS.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Predicted molecular weight: 38 kDa.
1/50 - 1/100.
The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
Expression level increases during S phase and drops in G2 phase (at protein level).
Protein modification; protein sumoylation.
Belongs to the ubiquitin-activating E1 family.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Information by UniProt
Activator of SUMO1 antibody
Western blot - Aos1 + Uba2 antibody (ab38434)
Anti-SAE1 antibody (ab38434) + Mouse heart tissue lysate
Predicted band size: 38 kDa Additional bands at: 59 kDa, 70 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Aos1 + Uba2 antibody (ab38434)
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with ab38434, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining
has not yet been referenced specifically in any publications.
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