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Read our guarantee »Products:Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Sumo
Anti-SAE1 antibody
See all SAE1 products (9) ...
Rabbit polyclonal to SAE1
ELISA, WB, IHC-Pmore details
Reacts with
Mouse, Human
Synthetic peptide conjugated to KLH, derived from within residues 10-30 at the C terminal of the last 50 amino acids of AOS1 (Human).
Mouse heart tissue lysate (WB). Human breast carcinoma tissue.
Liquid
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration information loading...
Protein G purified
This antibody is eluted out with both high and low pH buffers, neutralized immediately after elution then followed by dialysis against PBS.
Polyclonal
IgG
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ub-like Proteins
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Sumo
Our Abpromise guarantee covers the use of ab38434 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: 1/1000
WB: 1/100 - 1/500.Predicted molecular weight: 38 kDa.
IHC-P: 1/50 - 1/100.
The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
Expression level increases during S phase and drops in G2 phase (at protein level).
Protein modification; protein sumoylation.
Belongs to the ubiquitin-activating E1 family.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Nucleus.
Target information above from: UniProt accessionQ9UBE0
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Western blot - Aos1 + Uba2 antibody (ab38434)

Anti-SAE1 antibody (ab38434) + Mouse heart tissue lysate
Predicted band size : 38 kDa
Additional bands at : 59 kDa,70 kDa,75 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Aos1 + Uba2 antibody (ab38434)

Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with ab38434, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining
ab38434 has not yet been referenced specifically in any publications.
Publishing research using ab38434? Please let us know so that we can cite the reference in this datasheet
Concentration of lot no. is
Concentration not available for this lot.
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Anti-SAE1 antibody (ab38434) + Mouse heart tissue lysate
Predicted band size : 38 kDa
Additional bands at : 59 kDa,70 kDa,75 kDa. We are unsure as to the identity of these extra bands.

Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with ab38434, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining
0
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