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Products:Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Sumo
Overview
Product name
Anti-SAE1 antibody
See all SAE1 products (9) ...
Description
Goat polyclonal to SAE1
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide: MKGNGIVECLGPK, corresponding to C terminal amino acids 3374-346 of Human SAE1 .
MKGNGIVECL GPK
Positive control
Jurkat lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, 0.5% Tris-saline. pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ub-like Proteins
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Sumo
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Western blot - Anti-SAE1 antibody (ab5645)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab5645 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
Tested in peptide ELISA: antibody detection limit dilution 1/8000.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
Tissue specificity
Expression level increases during S phase and drops in G2 phase (at protein level).
Pathway
Protein modification; protein sumoylation.
Sequence similarities
Belongs to the ubiquitin-activating E1 family.
Post-translational
modifications
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ9UBE0
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AOS 1 antibody
- AOS1 antibody
- HSPC140 antibody
see all
Database links
- Entrez Gene: 10055 Human
- Omim: 613294 Human
- SwissProt: O95717 Human
- SwissProt: Q9UBE0 Human
- Unigene: 515500 Human
Anti-SAE1 antibody images:
Western blot - Anti-SAE1 antibody (ab5645)
Predicted band size : 40 kDa
ab5645 staining (1µg/ml) of Jurkat lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
References for Anti-SAE1 antibody (ab5645)
This product has been referenced in:
- Jones MCet al. Regulation of the SUMO pathway sensitizes differentiating human endometrial stromal cells to progesterone. Proc Natl Acad Sci U S A 103:16272-7 (2006).Read more (PubMed: 17053081) »
See 1 publication for this product
Publishing research using ab5645? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"