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Products:Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Sumo
Overview
Product name
Anti-SAE1 antibody
See all SAE1 products (9) ...
Description
Mouse monoclonal to SAE1
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant full length protein, corresponding to amino acids 1-347 of Human SAE1
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Isotype
IgG1
Light chain type
lambda
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ub-like Proteins
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Sumo
Applications
Show applications key
Our Abpromise guarantee covers the use of ab56957 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
WB: Use at a concentration of 1-5 µg/ml.
IHC-P: Use at a concentration of 3 µg/ml.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
Tissue specificity
Expression level increases during S phase and drops in G2 phase (at protein level).
Pathway
Protein modification; protein sumoylation.
Sequence similarities
Belongs to the ubiquitin-activating E1 family.
Post-translational
modifications
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ9UBE0
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AOS1 antibody
- HSPC140 antibody
- Sae1 antibody
see all
Database links
- Entrez Gene: 10055 Human
- Omim: 613294 Human
- SwissProt: O95717 Human
- SwissProt: Q9UBE0 Human
- Unigene: 515500 Human
Anti-SAE1 antibody images:
IHC-P - SAE1 antibody (ab56957)
SAE1 antibody (ab56957) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human adrenal gland.
Western blot - SAE1 antibody (ab56957)
SAE1 antibody (ab56957) at 1ug/lane + HeLa cell lysate at 25ug/lane.
References for Anti-SAE1 antibody (ab56957)
This product has been referenced in:
- Ribet Det al. Listeria monocytogenes impairs SUMOylation for efficient infection. Nature 464:1192-5 (2010). WB; Human.Read more (PubMed: 20414307) »
See 1 publication for this product
Publishing research using ab56957? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"