SAE1 antibody (ab56957)

Product Name 

SAE1 antibody

Product type 

Primary antibodies

Description 

Mouse monoclonal to SAE1

Immunogen 

Recombinant full length protein, corresponding to amino acids 1-347 of Human SAE1

Reacts with 
(species key)

Hu

Tested applications 
(see key)

IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: Use at a concentration of 1-5 µg/ml.
IHC-P: Use at a concentration of 3 µg/ml.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Nuclear

Research areas 

Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ub-like Proteins
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Sumo

Relevance 

Covalent attachment of one protein to another is one of the more prominent posttranslational modifications in respect to size and ubiquity to which eukaryotic proteins are subject. Ubiquitin is the most familiar of the protein modifiers and its activation and transfer to target proteins have been studied for over two decades. Recently a new group of ubiquitin like (Ubl) proteins has come to light. One of the most intriguing of them is SUMO (small ubiquitin like modifier, at 12 kDa), also known as Sentrin. SUMO family has been described in vertebrates: SUMO 1 and the closest homologs SUMO 2 and SUMO 3. SUMO 1 has been identified as a protein that is highly similar to ubiquitin and shown to conjugate to RanGAP1, PML, Sp200 and I kappa B alpha. The conjugation steps are thought to be similar to those of ubiquitination; Human Ubc9, which is homologous to the E2 enzyme for the ubiquitin conjugation step, was identified and shown to be necessary for the conjugation of SUMO 1 to its target protein. The recombinant proteins, Sua1p and hUba2p, formed a complex. In this complex, hUba2 bound SUMO 1 and this complex had the activity of the SUMO 1 activating enzyme. Furthermore, in an in vitro system, RanGAP1 was modified by SUMO 1 in the presence of Sua1p/Uba2p and hUbc9p, showing that the modification of SUMO 1 could be catalyzed by two enzyme steps, although ubiquitination usually requires three enzyme steps. These bindings implicate SUMO in the stabilization of the target proteins and/or their localization to subcellular complexes. SUMO research enters now an exciting phase with a promise to increase our understanding of how cells orchestrate the complexities of rapidly regulating protein level and activity.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Isotype 

IgG1

Light chain type 

lambda

Purity 

Protein G purified

Storage buffer 

Preservative: None
PBS, pH 7.2

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

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