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Products:Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Sumo
Overview
Product name
Anti-SAE1 antibody
See all SAE1 products (9) ...
Description
Rabbit polyclonal to SAE1
Specificity
ab58383 detects endogenous levels of total SAE1 protein.
Tested applications
ELISA, WB, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide derived from an internal sequence of SAE1.
Positive control
293 cell extracts.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab58383 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ub-like Proteins
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Sumo
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Western blot - SAE1 antibody (ab58383)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab58383 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/20000ELISA: 1/20000
WB: 1/500 - 1/1000.Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
ICC/IF: Use at an assay dependent dilution. (PubMed: 20498050)
Target
Function
The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
Tissue specificity
Expression level increases during S phase and drops in G2 phase (at protein level).
Pathway
Protein modification; protein sumoylation.
Sequence similarities
Belongs to the ubiquitin-activating E1 family.
Post-translational
modifications
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ9UBE0
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AOS 1 antibody
- AOS1 antibody
- FLJ3091 antibody
see all
Database links
- Entrez Gene: 10055 Human
- Entrez Gene: 56459 Mouse
- Entrez Gene: 308384 Rat
- Omim: 613294 Human
- SwissProt: O95717 Human
- SwissProt: Q9UBE0 Human
- SwissProt: Q9R1T2 Mouse
- SwissProt: Q6AXQ0 Rat
see all
Anti-SAE1 antibody images:
Western blot - SAE1 antibody (ab58383)
All lanes : Anti-SAE1 antibody (ab58383) at 1/500 dilution
Lane 1 : 293 cell extract
Lane 2 : 293 cell extract with immunising peptide
Predicted band size : 38 kDa
Observed band size : 38 kDa
References for Anti-SAE1 antibody (ab58383)
This product has been referenced in:
- Plant LDet al. One SUMO is sufficient to silence the dimeric potassium channel K2P1. Proc Natl Acad Sci U S A 107:10743-8 (2010). ICC/IF; Chinese Hamster.Read more (PubMed: 20498050) »
See 1 publication for this product
Publishing research using ab58383? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"