Anti-SAE2 / UBA2 antibody [4A3] (ab118404)

Overview

• Product nameAnti-SAE2 / UBA2 antibody [4A3]See all SAE2 / UBA2 primary antibodies ...
• Description
  Mouse monoclonal [4A3] to SAE2 / UBA2
• Tested applicationsWB, IHC-P, ICC/IF more details
• Species reactivity
  Reacts with: Rat, Human, Monkey
  Predicted to work with: Dog
• Immunogen

  Recombinant full length Human SAE2 / UBA2 produced in HEK293T cells (NP_005490).

• Positive controlHEK293T cell lysate transfected with pCMV6-ENTRY SAE2 / UBA2 cDNA; A549, COS7, HeLa, HepG2, HT29, Jurkat, MCF7, MDCK and PC12 cell extracts; COS7 cells transiently transfected with pCMV6-ENTRY SAE2 / UBA2 cDNA; Human endometrium, lymphoma, prostate and Human prostate carcinnoma tissues.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
• Storage bufferpH: 7.30
  Preservative: 0.02% Sodium azide
  Constituents: 48% PBS, 50% Glycerol, 1% BSA
• Concentration information loading...
• PurityProtein A purified
• Purification notesab118404 is purified from Mouse ascites fluid by affinity chromatography.
• Clonality Monoclonal
• Clone number4A3
• IsotypeIgG2a
• Research Areas
  • Cell Biology
  • Proteolysis / Ubiquitin
  • Proteasome / Ubiquitin
  • Sumo

Applications

Target

• FunctionThe heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
• PathwayProtein modification; protein sumoylation.
• Sequence similaritiesBelongs to the ubiquitin-activating E1 family.
• Cellular localizationNucleus.

Target information above from: UniProt accession Q9UBT2 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 10054 Human
  • Entrez Gene: 308508 Rat
  • Omim: 613295 Human
  • SwissProt: Q9UBT2 Human
  • Unigene: 631580 Human
  • Unigene: 198150 Rat

• Alternative names
  Anthracycline associated resistance ARX antibodyAnthracycline-associated resistance ARX antibodyARX antibody

  FLJ13058 antibodyHRIHFB2115 antibodySAE 2 antibodySAE2 antibodySAE2_HUMAN antibodySUMO 1 activating enzyme subunit 2 antibodySUMO activating enzyme subunit 2 antibodySUMO-activating enzyme subunit 2 antibodyUBA2 antibodyUBA2 ubiquitin activating enzyme E1 homolog antibodyUbiquitin like 1 activating enzyme E1B antibodyUbiquitin like modifier activating enzyme 2 antibodyUbiquitin-like 1-activating enzyme E1B antibodyUBLE1B antibody

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Anti-SAE2 / UBA2 antibody [4A3] images

• 

  Western blot - Anti-SAE2 / UBA2 antibody [4A3] (ab118404)
  All lanes : Anti-SAE2 / UBA2 antibody [4A3] (ab118404) at 1/500 dilution
  
  Lane 1 : HepG2 cell extract
  Lane 2 : HeLA cell extract
  Lane 3 : HT29 cell extract
  Lane 4 : A549 cell extract
  Lane 5 : COS7 cell extract
  Lane 6 : Jurkat cell extract
  Lane 7 : MDCK cell extract
  Lane 8 : PC12 cell extract
  Lane 9 : MCF7 cell extract
  
  Lysates/proteins at 35 µg per lane.
  
  
  Predicted band size : 71 kDa
  
• 

  Western blot - Anti-SAE2 / UBA2 antibody [4A3] (ab118404)
  All lanes : Anti-SAE2 / UBA2 antibody [4A3] (ab118404) at 1/500 dilution
  
  Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control cDNA
  Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY SAE2 / UBA2 cDNA
  
  Lysates/proteins at 5 µg per lane.
  
  
  Predicted band size : 71 kDa
  
• 

  Immunocytochemistry/ Immunofluorescence - Anti-SAE2 / UBA2 antibody [4A3] (ab118404)
  ab118404 at 1/100 dilution staining SAE2 / UBA2 in COS7 cells transiently transfected by pCMV6-ENTRY SAE2 / UBA2 by Immunofluorescence.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAE2 / UBA2 antibody [4A3] (ab118404)
  ab118404 at 1/150 dilution staining SAE2 / UBA2 in Paraffin-embedded Human endometrium tissue by Immunohistochemistry.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAE2 / UBA2 antibody [4A3] (ab118404)
  ab118404 at 1/150 dilution staining SAE2 / UBA2 in Paraffin-embedded Human Prostate tissue by Immunohistochemistry.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAE2 / UBA2 antibody [4A3] (ab118404)
  ab118404 at 1/50 dilution staining SAE2 / UBA2 in Paraffin-embedded Human Prostate carcinoma tissue by Immunohistochemistry.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAE2 / UBA2 antibody [4A3] (ab118404)
  ab118404 at 1/150 dilution staining SAE2 / UBA2 in Paraffin-embedded Human Lymphoma tissue by Immunohistochemistry.