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Anti-SAFB antibody [4i59] (ab18086)

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Overview

Product name

Anti-SAFB antibody [4i59]
See all SAFB products (5) ...

Description

Mouse monoclonal [4i59] to SAFB

Specificity

Ab18086 recognizes HET/SAFB, Mr 130kDa; and a protein, Mr 34kDa.

Tested applications

WB, IP, ICC, IHC-Pmore details

Cross reactivity

Reacts with

Human

Does not react with

Mouse

Immunogen

Synthetic peptide: CPEARDKEDGRKF, corresponding to amino acids 345 to 357 of SAFB.

CPEARDKEDG RKF

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Epitope

The immunizing sequence has 8/11 identical amino acids in mouse and human transcription factor tbx2 (T-box protein 2).

Positive control

An independent laboratory has shown HET/SAFB to be immunoprecipitated from MCF-7 and HepG2 cell lysates.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.05% Sodium Azide
Constituents: 30% Glycerol, 0.15M Sodium chloride, 0.1M Tris glycine. pH 7.4

Concentration

Concentration information loading...

Purity

Protein G purified

Clonality

Monoclonal

Clone number

4i59

Isotype

IgG

Research areas

Epigenetics and Nuclear Signaling >> Chromosome Structure >> Scaffold Proteins

Applications

Show applications key

Our Abpromise guarantee covers the use of ab18086 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Binds to scaffold/matrix attachment region (S/MAR) DNA and forms a molecular assembly point to allow the formation of a 'transcriptosomal' complex (consisting of SR proteins and RNA polymerase II) coupling transcription and RNA processing (By similarity). Can function as an estrogen receptor corepressor and can also bind to the HSP27 promoter and decrease its transcription. Can inhibit cell proliferation.

Tissue specificity

Ubiquitous. Expressed at high levels in the CNS and at low levels in the liver. Expressed in a wide number of breast cancer cell lines.

Sequence similarities

Contains 1 RRM (RNA recognition motif) domain.
Contains 1 SAP domain.

Cellular localization

Nucleus.

Target information above from: UniProt accessionQ15424 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • DKFZp779C1727 antibody
  • glutathione S transferase fusion protein antibody
  • HAP antibody
  • HET antibody
  • HSP27 ERE TATA binding protein antibody
  • HSP27 ERE-TATA-binding protein antibody
  • HSP27 estrogen response element-TATA box-binding protein antibody
  • SAF B antibody
  • SAF-B antibody
  • SAF-B1 antibody
  • SAFB 1 antibody
  • SAFB antibody
  • SAFB1 antibody
  • SAFB1_HUMAN antibody
  • scaffold attachment factor B1 antibody
see all

Database links

References for Anti-SAFB antibody [4i59] (ab18086)

ab18086 has not yet been referenced specifically in any publications.

Publishing research using ab18086? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"