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Product Name
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SAFB antibody [4i59]
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See all SAFB antibodies (3)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [4i59] to SAFB
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Immunogen
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Synthetic peptide: CPEARDKEDGRKF, corresponding to amino acids 345 to 357 of SAFB.
BLAST 'CPEARDKEDGRKF' with  or 
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Reacts with
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Hu
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Does not react with
(species key)
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Ms
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Specificity
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Ab18086 recognizes HET/SAFB, Mr 130kDa; and a protein, Mr 34kDa.
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Epitope
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The immunizing sequence has 8/11 identical amino acids in mouse and human transcription factor tbx2 (T-box protein 2).
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Tested applications
(see key)
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ICC, IHC-P, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC: Use at an assay dependent dilution.
IHC-P: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: Use at a concentration of 0.5 µg/ml. Predicted molecular weight: 102 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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An independent laboratory has shown HET/SAFB to be immunoprecipitated from MCF-7 and HepG2 cell lysates.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Chromosome Structure >> Scaffold Proteins
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Relevance
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SAFB (Scaffold Attachment Factor-B) is a member of a subset of nuclear matrix proteins that mediate the attachment of chromatin to nuclear protein complexes however it is not part of the nuclear matrix.
SAFB is ubiquitously expressed and appears to be a multifunctional protein involved in chromatin organization, mRNA processing and transcriptional regulation. It is expressed at high levels in the CNS and at low levels in the liver. Also expressed in a wide number of breast cancer cell lines.
SAFB has been demonstrated to interact with and function as a corepressor with many nuclear receptors such as estrogen receptor-alpha, PPAR-gamma, and FXR-alpha.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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4i59
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Isotype
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IgG
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Purity
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Protein G purified
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Storage buffer
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Preservative: 0.05% Sodium Azide
Constituents: 30% Glycerol, 0.15M Sodium chloride, 0.1M Tris glycine. pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this SAFB antibody [4i59] is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to SAFB
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SAFB antibody [4i59] - more information
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Customer reviews (feedback) regarding SAFB antibody [4i59] |
Customer FAQs regarding SAFB antibody [4i59] |
Protocols for SAFB antibody [4i59] |
Price and availability of products related to SAFB antibody [4i59] |
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SAFB antibody [4i59] - related products:
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SAFB antibody [4i59] - other tools:
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