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ab8060 has been referenced in 7 publications.
Publishing research using ab8060? Please let us know so that we can cite the reference in this datasheet
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Anti-SAFB antibody [6F7] (ab8060) at 1/5000 dilution + 293T cells - whole cell lysate at 30 µg
Secondary
HRP conjugated Sheep anti-mouse IgG
developed using the ECL technique
Performed under reducing conditions.
Observed band size : 130 kDa (why is the actual band size different from the predicted?)
Additional bands at : 60 kDa (possible non-specific binding).
Exposure time : 1 minute
This image is courtesy of an Abreview submitted by Dr Stoil Dmitrov
ab8060 at 1/500 staining human U2OS cells (osteosarcoma cell line) by ICC/IF. The cells were paraformaldehyde fixed and blocked with BSA prior to incubation with the antibody for 30 minutes. A FITC conjugated goat anti-mouse antibody was used as the secondary (right hand panel). The cells were also stained with DAPI (left hand panel).
This image is courtesy of an Abreview submitted by Dr Stoil Dmitrov
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