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Read our guarantee »Anti-SAFB antibody [6F7]
See all SAFB products (5) ...
Mouse monoclonal [6F7] to SAFB
WB, IP, ICC/IFmore details
Reacts with
Mouse, Human
A synthetic peptide corresponding to amino acids 345-357(PEARDSKEDGRKF) of the human HET protein.
PEARDKEDGR KF
HeLa, MCF-7 cells.
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Phosphate buffered saline
Concentration information loading...
Protein G purified
1mg/ml of antibody purified from hybridoma cell culture supernatant byprotein G chromatography to at least 95% homogeneity as determined by SDS-PAGE chromatography. Prepared in 10mM PBS, pH 7.4.
Monoclonal
6F7
NS1
IgG1
kappa
Epigenetics and Nuclear Signaling >> Chromosome Structure >> Scaffold Proteins
Our Abpromise guarantee covers the use of ab8060 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 2 µg/ml.
IP: Use at an assay dependent dilution.
ICC/IF: Use at an assay dependent dilution.
Binds to scaffold/matrix attachment region (S/MAR) DNA and forms a molecular assembly point to allow the formation of a 'transcriptosomal' complex (consisting of SR proteins and RNA polymerase II) coupling transcription and RNA processing (By similarity). Can function as an estrogen receptor corepressor and can also bind to the HSP27 promoter and decrease its transcription. Can inhibit cell proliferation.
Ubiquitous. Expressed at high levels in the CNS and at low levels in the liver. Expressed in a wide number of breast cancer cell lines.
Contains 1 RRM (RNA recognition motif) domain.
Contains 1 SAP domain.
Nucleus.
Target information above from: UniProt accessionQ15424
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Western blot - SAFB antibody [6F7] (ab8060)
![Western blot - SAFB antibody [6F7] (ab8060)](/ps/datasheet/Images/8/ab8060/ab8060_1.jpg)
Anti-SAFB antibody [6F7] (ab8060) at 1/5000 dilution + 293T cells - whole cell lysate at 30 µg
Secondary
HRP conjugated Sheep anti-mouse IgG
developed using the ECL technique
Performed under reducing conditions.
Observed band size : 130 kDa (why is the actual band size different from the predicted?)
Additional bands at : 60 kDa (possible non-specific binding).
Exposure time : 1 minute
This image is courtesy of an Abreview submitted by Dr Stoil Dmitrov
Immunocytochemistry/ Immunofluorescence - SAFB antibody [6F7] (ab8060)
![Immunocytochemistry/ Immunofluorescence - SAFB antibody [6F7] (ab8060)](/ps/datasheet/Images/8/ab8060/ab8060_2.jpg)
ab8060 at 1/500 staining human U2OS cells (osteosarcoma cell line) by ICC/IF. The cells were paraformaldehyde fixed and blocked with BSA prior to incubation with the antibody for 30 minutes. A FITC conjugated goat anti-mouse antibody was used as the secondary (right hand panel). The cells were also stained with DAPI (left hand panel).
This image is courtesy of an Abreview submitted by Dr Stoil Dmitrov
This product has been referenced in:
See all 7 publications for this product
Publishing research using ab8060? Please let us know so that we can cite the reference in this datasheet
Concentration of lot no. is
Concentration not available for this lot.
Find concentration of your lot:
![Western blot - SAFB antibody [6F7] (ab8060)](/ps/datasheet/Images/8/ab8060/ab8060_1.jpg)
Anti-SAFB antibody [6F7] (ab8060) at 1/5000 dilution + 293T cells - whole cell lysate at 30 µg
Secondary
HRP conjugated Sheep anti-mouse IgG
developed using the ECL technique
Performed under reducing conditions.
Observed band size : 130 kDa (why is the actual band size different from the predicted?)
Additional bands at : 60 kDa (possible non-specific binding).
Exposure time : 1 minute
This image is courtesy of an Abreview submitted by Dr Stoil Dmitrov
![Immunocytochemistry/ Immunofluorescence - SAFB antibody [6F7] (ab8060)](/ps/datasheet/Images/8/ab8060/ab8060_2.jpg)
ab8060 at 1/500 staining human U2OS cells (osteosarcoma cell line) by ICC/IF. The cells were paraformaldehyde fixed and blocked with BSA prior to incubation with the antibody for 30 minutes. A FITC conjugated goat anti-mouse antibody was used as the secondary (right hand panel). The cells were also stained with DAPI (left hand panel).
This image is courtesy of an Abreview submitted by Dr Stoil Dmitrov
3
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