Feedback Form

Western blot - SAFB antibody [6F7] (ab8060)
Western blot - SAFB antibody [6F7] (ab8060)
(enlarge)


Immunocytochemistry/ Immunofluorescence - SAFB antibody [6F7] (ab8060)
Immunocytochemistry/ Immunofluorescence - SAFB antibody [6F7] (ab8060)
(enlarge)


Product Name 

SAFB antibody [6F7]

 

See all SAFB antibodies (3)...

Product type 

Primary antibodies

Description 

Mouse monoclonal [6F7] to SAFB

Immunogen 

A synthetic peptide corresponding to amino acids 345-357(PEARDSKEDGRKF) of the human HET protein.
BLAST 'PEARDKEDGRKF' with BLAST the sequence with ExPASy or BLAST the sequence with NCBI

Reacts with 
(species key)

Hu, Ms

Tested applications 
(see key)

ICC/IF, IP, WB


Abreviews 

Customer reviews
(see key)


Read about Abreviews
(Read all Abreviews)

Submit an Abreview and earn up to 220 Abpoints by reviewing this product

• ICC/IF

2 Abreviews

- average rating: 5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)

• WB

1 Abreview

- average rating: 4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)


ICC/IF
(read Abreview)


ICC/IF
(read Abreview)


WB
(read Abreview)


Application notes 
(see key)

Recommended dilutions
ICC/IF: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: Use at a concentration of 1 - 2 µg/ml.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

HeLa, MCF-7 cells.

Cellular localization 

Nuclear

Research areas 

Chromatin and Nuclear Signaling >> Chromosome Structure >> Scaffold Proteins

Relevance 

SAFB (Scaffold Attachment Factor-B) is a member of a subset of nuclear matrix proteins that mediate the attachment of chromatin to nuclear protein complexes however it is not part of the nuclear matrix.

SAFB is ubiquitously expressed and appears to be a multifunctional protein involved in chromatin organization, mRNA processing and transcriptional regulation. It is expressed at high levels in the CNS and at low levels in the liver. Also expressed in a wide number of breast cancer cell lines.

SAFB has been demonstrated to interact with and function as a corepressor with many nuclear receptors such as estrogen receptor-alpha, PPAR-gamma, and FXR-alpha.



 

Alternative names SAFB antibody [6F7] (ab8060)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

6294    (Human)
224903    (Mouse)

Omim

    

602895    (Human)

SwissProt

    

Q15424    (Human)

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

6F7

Myeloma 

NS1

Isotype 

IgG1

Light chain type 

kappa

Purity 

Protein G purified

Storage buffer 

Phosphate buffered saline

Purification notes 

1mg/ml of antibody purified from hybridoma cell culture supernatant byprotein G chromatography to at least 95% homogeneity as determined by SDS-PAGE chromatography. Prepared in 10mM PBS, pH 7.4.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this SAFB antibody [6F7] is on this datasheet. But please do contact us if you would like any reassurance!


See below for SAFB antibody [6F7] images, references, products related to ab8060 and other tools.

 

SAFB antibody [6F7] images:

Product image  Western blot - SAFB antibody [6F7] (ab8060)

Western blot - SAFB antibody [6F7] (ab8060)

SAFB antibody [6F7] (ab8060) at 1/5000 dilution + 293T cells - whole cell lysate at 30 µg

Secondary
HRP conjugated Sheep anti-mouse IgG
developed using the ECL technique

Performed under reducing conditions.

Observed band size : 130 kDa (why is the actual band size different from the predicted?)
Additional bands at : 60 kDa (possible non-specific binding).

Exposure time : 1 minute

This image is courtesy of an Abreview submitted by Dr Stoil Dmitrov

See Abreview

 

Product image  Immunocytochemistry/ Immunofluorescence - SAFB antibody [6F7] (ab8060)

Immunocytochemistry/ Immunofluorescence - SAFB antibody [6F7] (ab8060)

ab8060 at 1/500 staining human U2OS cells (osteosarcoma cell line) by ICC/IF. The cells were paraformaldehyde fixed and blocked with BSA prior to incubation with the antibody for 30 minutes. A FITC conjugated goat anti-mouse antibody was used as the secondary (right hand panel). The cells were also stained with DAPI (left hand panel).

This image is courtesy of an Abreview submitted by Dr Stoil Dmitrov

See Abreview

 

References for SAFB antibody [6F7] (ab8060)

Specific publication references   This product has been used in: (two most recent references)

Schimmel J et al. The ubiquitin-proteasome system is a key component of the SUMO-2/3 cycle. Mol Cell Proteomics : (2008). WB. PubMed: 18565875

Townson SM et al. HET/SAF-B overexpression causes growth arrest and multinuclearity and is associated with aneuploidy in human breast cancer. Clin Cancer Res 6:3788-96 (2000). PubMed: 10999774


See all 3 publication references for this product.


If you publish research using ab8060 please let us know so that we can cite the reference on this datasheet.

 

Search PubMed (MEDLINE) for references to SAFB

 

SAFB antibody [6F7] - more information

Customer reviews (feedback) regarding SAFB antibody [6F7]

Customer FAQs regarding SAFB antibody [6F7]

Protocols for SAFB antibody [6F7]

Price and availability of products related to SAFB antibody [6F7]

 

SAFB antibody [6F7] - related products:

Products for Western blot

Loading controls

Lysates

 

Compatible Secondaries for SAFB antibody [6F7]

ab2891

  

HRP polymer antibody

ab6563

  

Cy5 ® antibody

ab6726

  

Texas Red ® antibody

ab6728

  

HRP antibody

ab6729

  

Alkaline Phosphatase antibody

ab6785

  

FITC antibody

ab6788

  

Biotin antibody

ab7002

  

Phycoerythrin antibody

ab7018

  

Agarose antibody

ab27229

  

15nm Gold antibody

ab27230

  

10nm Gold antibody

ab27231

  

15nm Gold antibody

ab27233

  

5nm Gold antibody

ab27241

  

10nm Gold antibody

ab27242

  

20nm Gold antibody

ab27243

  

40nm Gold antibody

ab27244

  

5nm Gold antibody

ab60313

  

Chromeo™ 488 antibody

ab60316

  

Chromeo™ 546 antibody

ab60318

  

Chromeo™ 642 antibody

 

Other SAFB Antibodies

See all SAFB antibodies (3)...

 

SAFB antibody [6F7] - other tools:

Contact Abcam with a Technical Enquiry about SAFB antibody [6F7]

 

All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

©1998-2010 Abcam plc. All rights reserved.