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Western blot - SAFB antibody [6F7] (ab8060)
![Western blot - SAFB antibody [6F7] (ab8060)](/ps/datasheet/images/ab8060_1.jpg)
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Immunocytochemistry/ Immunofluorescence - SAFB antibody [6F7] (ab8060)
![Immunocytochemistry/ Immunofluorescence - SAFB antibody [6F7] (ab8060)](/ps/datasheet/images/ab8060_2.jpg)
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Product Name
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SAFB antibody [6F7]
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See all SAFB antibodies (3)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [6F7] to SAFB
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Immunogen
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A synthetic peptide corresponding to amino acids 345-357(PEARDSKEDGRKF) of the human HET protein.
BLAST 'PEARDKEDGRKF' with  or 
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Reacts with
(species key)
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Hu, Ms
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Tested applications
(see key)
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ICC/IF, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: Use at a concentration of 1 - 2 µg/ml.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HeLa, MCF-7 cells.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Chromosome Structure >> Scaffold Proteins
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Relevance
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SAFB (Scaffold Attachment Factor-B) is a member of a subset of nuclear matrix proteins that mediate the attachment of chromatin to nuclear protein complexes however it is not part of the nuclear matrix.
SAFB is ubiquitously expressed and appears to be a multifunctional protein involved in chromatin organization, mRNA processing and transcriptional regulation. It is expressed at high levels in the CNS and at low levels in the liver. Also expressed in a wide number of breast cancer cell lines.
SAFB has been demonstrated to interact with and function as a corepressor with many nuclear receptors such as estrogen receptor-alpha, PPAR-gamma, and FXR-alpha.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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6F7
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Myeloma
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NS1
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Isotype
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IgG1
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Light chain type
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kappa
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Purity
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Protein G purified
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Storage buffer
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Phosphate buffered saline
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Purification notes
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1mg/ml of antibody purified from hybridoma cell culture supernatant byprotein G chromatography to at least 95% homogeneity as determined by SDS-PAGE chromatography. Prepared in 10mM PBS, pH 7.4.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this SAFB antibody [6F7] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for SAFB antibody [6F7] images, references, products related to ab8060 and other tools.
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SAFB antibody [6F7] images:
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 Western blot - SAFB antibody [6F7] (ab8060)
SAFB antibody [6F7] (ab8060) at 1/5000 dilution + 293T cells - whole cell lysate at 30 µg
Secondary
HRP conjugated Sheep anti-mouse IgG
developed using the ECL technique
Performed under reducing conditions.
Observed band size : 130 kDa (why is the actual band size different from the predicted?)
Additional bands at : 60 kDa (possible non-specific binding).
Exposure time : 1 minute This image is courtesy of an Abreview submitted by Dr Stoil Dmitrov
See Abreview
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Immunocytochemistry/ Immunofluorescence - SAFB antibody [6F7] (ab8060)
ab8060 at 1/500 staining human U2OS cells (osteosarcoma cell line) by ICC/IF. The cells were paraformaldehyde fixed and blocked with BSA prior to incubation with the antibody for 30 minutes. A FITC conjugated goat anti-mouse antibody was used as the secondary (right hand panel). The cells were also stained with DAPI (left hand panel). This image is courtesy of an Abreview submitted by Dr Stoil Dmitrov
See Abreview
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References for SAFB antibody [6F7] (ab8060)
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This product has been used in: (two most recent references)
Schimmel J et al. The ubiquitin-proteasome system is a key component of the SUMO-2/3 cycle. Mol Cell Proteomics : (2008). WB. PubMed: 18565875
Townson SM et al. HET/SAF-B overexpression causes growth arrest and multinuclearity and is associated with aneuploidy in human breast cancer. Clin Cancer Res 6:3788-96 (2000). PubMed: 10999774
See all 3 publication references for this product.
If you publish research using ab8060 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to SAFB
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SAFB antibody [6F7] - more information
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Customer reviews (feedback) regarding SAFB antibody [6F7] |
Customer FAQs regarding SAFB antibody [6F7] |
Protocols for SAFB antibody [6F7] |
Price and availability of products related to SAFB antibody [6F7] |
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SAFB antibody [6F7] - related products:
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SAFB antibody [6F7] - other tools:
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Contact Abcam with a Technical Enquiry about SAFB antibody [6F7] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Western blot - SAFB antibody [6F7] (ab8060)](/index.html?datasheet=8060#ab8060_1.jpg)
![Immunocytochemistry/ Immunofluorescence - SAFB antibody [6F7] (ab8060)](/index.html?datasheet=8060#ab8060_2.jpg)
