SAG (Smo agonist), Smoothened agonist (ab142160)
Key features and details
- Cell-permeable, selective, potent Smoothened agonist
- CAS Number: 912545-86-9
- Purity: > 98%
- Soluble in DMSO to 100 mM
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
SAG (Smo agonist), Smoothened agonist -
Description
Cell-permeable, selective, potent Smoothened agonist -
Biological description
Cell-permeable, selective, potent Smoothened agonist (EC50 = 3 nM). Binds the Smo heptahelical bundle that antagonizes cyclopamine (ab120392) action. Active in vitro and in vivo.
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Purity
> 98% -
CAS Number
912545-86-9 -
Chemical structure
Properties
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Chemical name
3-Chloro-N-[trans-4-(methylamino)cyclohexyl]-N-[[3-(4-pyridinyl)phenyl]methyl]benzo[b]thiophene-2-carboxamide -
Molecular weight
490.06 -
Molecular formula
C28H28ClN3OS -
PubChem identifier
5284330 -
Storage instructions
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in DMSO to 100 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
CN[C@H]1CC[C@@H](CC1)N(Cc2ccc(cc2)c3ccncc3)C(=O)c5sc4ccccc4c5Cl -
Source
Synthetic
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Research areas
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (9)
ab142160 has been referenced in 9 publications.
- Yamakawa D et al. Cilia-Mediated Insulin/Akt and ST2/JNK Signaling Pathways Regulate the Recovery of Muscle Injury. Adv Sci (Weinh) 10:e2202632 (2022). PubMed: 36373718
- May EA et al. Time-resolved proteomics profiling of the ciliary Hedgehog response. J Cell Biol 220:N/A (2021). PubMed: 33856408
- Petsouki E et al. FBW7 couples structural integrity with functional output of primary cilia. Commun Biol 4:1066 (2021). PubMed: 34518642
- Yamazoe T et al. Roles of TOG and jelly-roll domains of centrosomal protein CEP104 in its functions in cilium elongation and Hedgehog signaling. J Biol Chem 295:14723-14736 (2020). PubMed: 32820051
- Tessier S et al. NFAT5/TonEBP controls early acquisition of notochord phenotypic markers, collagen composition, and sonic hedgehog signaling during mouse intervertebral disc embryogenesis. Dev Biol 455:369-381 (2019). PubMed: 31301300
- Kim BH et al. Deficiency of calpain-6 inhibits primary ciliogenesis. BMB Rep 52:619-624 (2019). PubMed: 31619317
- Coulter ME et al. The ESCRT-III Protein CHMP1A Mediates Secretion of Sonic Hedgehog on a Distinctive Subtype of Extracellular Vesicles. Cell Rep 24:973-986.e8 (2018). PubMed: 30044992
- Meerang M et al. Antagonizing the Hedgehog Pathway with Vismodegib Impairs Malignant Pleural Mesothelioma Growth In Vivo by Affecting Stroma. Mol Cancer Ther 15:1095-105 (2016). PubMed: 26839306
- Xing Y et al. Liver X receptor a is essential for the capillarization of liver sinusoidal endothelial cells in liver injury. Sci Rep 6:21309 (2016). PubMed: 26887957