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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
Overview
Product name
Anti-SALL1 antibody
See all SALL1 products (3) ...
Description
Rabbit polyclonal to SALL1
Tested applications
IHC-P, WBmore details
Cross reactivity
Reacts with
Mouse
Predicted to work with
Chicken, Human, Fruit fly (Drosophila melanogaster)
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1300 to the C-terminus of Human SALL1.
(Peptide available as ab31903.)
Positive control
The antibody gave a positive signal in E14Tg2a cell lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab31526 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at an assay dependent dilution.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 130 kDa (predicted molecular weight: 140 kDa).
Target
Relevance
SALL1, a member of the C2H2-type zinc-finger protein family is responsible for transcriptionally repressing the nucleosome remodelling and deacetylase (NuRD) complex. The NuRD complex is involved in facilitating global transcriptional repression and regulation of specific developmental processes. Mutations in the N-terminal of SALL1 leads to Townes-Brocks syndrome(TBS), which is a rare, autosomal dominant malformation syndrome with a combination of imperforate anus, triphalangeal and supernumerary thumbs, malformed ears and sensorineural hearing loss.
Cellular localization
Nuclear
Alternative names
- HSal1 antibody
- Sal like protein 1 antibody
- Sal1 antibody
see all
Database links
- Entrez Gene: 395446 Chicken
- Entrez Gene: 6299 Human
- Entrez Gene: 58198 Mouse
- Omim: 602218 Human
- SwissProt: Q9NSC2 Human
- SwissProt: Q9ER74 Mouse
- Unigene: 135787 Human
- Unigene: 214361 Mouse
Anti-SALL1 antibody images:
Western blot - SALL1 antibody (ab31526)
Anti-SALL1 antibody (ab31526) at 1 µg/ml + E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size : 140 kDa
Observed band size : 130 kDa (why is the actual band size different from the predicted?)
Additional bands at : 200 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 2 minutes
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SALL1 antibody (ab31526)
ab31526 staining SALL1 in metanephric blastema cells, murine kidney tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Image from Di Giovanni V et al, Development. 2011 Jul;138(13):2717-27. Epub 2011 May 25, Fig 4. DOI 10.1242/dev.059030/-/DC1
References for Anti-SALL1 antibody (ab31526)
This product has been referenced in:
- Di Giovanni Vet al. Alk3 controls nephron number and androgen production via lineage-specific effects in intermediate mesoderm. Development 138:2717-27 (2011). IHC-P; Mouse.Read more (PubMed: 21613322) »
See 1 publication for this product
Publishing research using ab31526? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"