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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab3608? Please let us know so that we can cite the reference in this datasheet
ab3608 has been referenced in 2 publications.
- Zhang Z et al. Synthesis and characterization of pseudocantharidins, novel phosphatase modulators that promote the inclusion of exon 7 into the survival of motoneuron (SMN) pre-mRNA. J Biol Chem : (2011). WB; Human. PubMed: 21220421
- Yoshimoto R et al. Isolation and characterization of post-splicing lariat-intron complexes. Nucleic Acids Res : (2008). WB; Human. PubMed: 19103666
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - SAP130 antibody (ab3608)
Anti-SAP130 antibody (ab3608) at 0.1 µg/ml + HeLa cells, nuclear lysate at 30 µg
Predicted band size : 136 kDa
Observed band size : 150 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-SAP130 antibody(ab3608)
IHC image of ab3608 staining in human normal cervix formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab3608, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
SAP130 antibody for WB in Mouse (3608)
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