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Overview
Product name
Anti-SAP130 antibody
See all SAP130 products (4) ...
Description
Goat polyclonal to SAP130
Tested applications
IHC-P, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Dog
Immunogen
Synthetic peptide: VSKKLEDIRTRYAF, corresponding to C terminal amino acids 1204-1217 of Human SAP130.
C-VSKKLEDI RTRYAF
Positive control
HeLa or Raji Lysate.
General notes
Principal Names – SF3B3; RSE1; SAP130; SF3B130; SF3b130; KIAA0017; splicing factor 3b, subunit 3, 130kDa; spliceosome-associated protein 130; splicing factor 3b, subunit 3, 130 kDa.
Official Gene Symbol - SF3B3.
GenBank Accession Number – NP_036558.
LocusLink ID - 23450 (human); 101943 (mouse).
Gene Ontology terms - pre-mRNA splicing factor activity; small nuclear ribonucleoprotein; mRNA processing; mRNA splicing; protein complex assembly; spliceosome complex.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab3608 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use a concentration of 5 µg/mlPerform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB: Use a concentration of 0.1 - 0.3 µg/ml.Detects a band of approximately 150 kDa (predicted molecular weight: 136 kDa).
Target
Function
Acts as a transcriptional repressor. May function in the assembly and/or enzymatic activity of the mSin3A corepressor complex or in mediating interactions between the complex and other regulatory complexes.
Tissue specificity
Expressed in various cancer cell ines.
Sequence similarities
Belongs to the SAP130 family.
Domain
The N-terminus may interact with a transcriptional coactivator.
The C-terminus may interact with HDAC-dependent and HDAC-independent corepressors.
Post-translational
modifications
Acetylated.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ9H0E3
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 130 kDa Sin3-associated polypeptide antibody
- FLJ12761 antibody
- Histone deacetylase complex subunit SAP130 antibody
see all
Database links
- Entrez Gene: 79595 Human
- Entrez Gene: 269003 Mouse
- Entrez Gene: 307527 Rat
- Omim: 609697 Human
- SwissProt: Q15393 Human
- SwissProt: Q9H0E3 Human
- SwissProt: Q8BIH0 Mouse
- Unigene: 32995 Human
see all
Anti-SAP130 antibody images:
Western blot - SAP130 antibody (ab3608)
Anti-SAP130 antibody (ab3608) at 0.1 µg/ml + HeLa cells, nuclear lysate at 30 µg
Predicted band size : 136 kDa
Observed band size : 150 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-SAP130 antibody(ab3608)
IHC image of ab3608 staining in human normal cervix formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab3608, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
References for Anti-SAP130 antibody (ab3608)
This product has been referenced in:
- Zhang Zet al. Synthesis and characterization of pseudocantharidins, novel phosphatase modulators that promote the inclusion of exon 7 into the survival of motoneuron (SMN) pre-mRNA. J Biol Chem : (2011). WB; Human.Read more (PubMed: 21220421) »
- Yoshimoto Ret al. Isolation and characterization of post-splicing lariat-intron complexes. Nucleic Acids Res : (2008). WB; Human.Read more (PubMed: 19103666) »
See all 2 publications for this product
Publishing research using ab3608? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"