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Western blot - SAP130 antibody (ab3608)
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Western blot - SAP130 antibody (ab3608)
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Product Name
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SAP130 antibody
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See all SAP130 antibodies (2)...
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Product type
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Primary antibodies
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Description
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Goat polyclonal to SAP130
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Immunogen
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Synthetic peptide: VSKKLEDIRTRYAF, corresponding to C terminal amino acids 1204-1217 of Human SAP130.
BLAST 'C-VSKKLEDIRTRYAF' with  or 
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Reacts with
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Hu
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Predicted to react
(species key)
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Ms, Rat, Dog
(Due to sequence homology)
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/8000.
WB: Use at a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 135 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HeLa or Raji Lysate.
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Research areas
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Chromatin and Nuclear Signaling >> Chromatin Binding Proteins >> DNA / RNA binding
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Relevance
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SAP130 is a member of the RNA splicing machinery. It is a component of splicing factor SF3b that associates with U2 snRNP and is recruited to prespliceosomal complexes.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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IgG fraction
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Storage buffer
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Tris saline, 0.02% Na azide, pH7.3 with 0.5% BSA Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Notes
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Principal Names – SF3B3; RSE1; SAP130; SF3B130; SF3b130; KIAA0017; splicing factor 3b, subunit 3, 130kDa; spliceosome-associated protein 130; splicing factor 3b, subunit 3, 130 kDa.
Official Gene Symbol - SF3B3.
GenBank Accession Number – NP_036558.
LocusLink ID - 23450 (human); 101943 (mouse).
Gene Ontology terms - pre-mRNA splicing factor activity; small nuclear ribonucleoprotein; mRNA processing; mRNA splicing; protein complex assembly; spliceosome complex.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this SAP130 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for SAP130 antibody images, references, products related to ab3608 and other tools.
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SAP130 antibody images:
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 Western blot - SAP130 antibody (ab3608)
Predicted band size : 135 kDa
ab3608 at a 0.5µg/ml concentration staining ~140 kDa SAP 130 in Raji cell lysate (RIPA buffer, 30µg total protein per lane) by Western blot. 1 hour primary incubation, ECL detection.
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References for SAP130 antibody (ab3608)
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This product has been used in:
Yoshimoto R et al. Isolation and characterization of post-splicing lariat-intron complexes. Nucleic Acids Res : (2008). WB; Human. PubMed: 19103666
If you publish research using ab3608 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to SAP130
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SAP130 antibody - more information
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Customer reviews (feedback) regarding SAP130 antibody |
Customer FAQs regarding SAP130 antibody |
Protocols for SAP130 antibody |
Price and availability of products related to SAP130 antibody |
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SAP130 antibody - related products:
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SAP130 antibody - other tools:
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Contact Abcam with a Technical Enquiry about SAP130 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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