Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Overview
Product name
Anti-SAP130 antibody
See all SAP130 products (4) ...
Description
Rabbit polyclonal to SAP130
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Recombinant fragment, corresponding to amino acids 819-1217 of Human SAP130
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 50% Glycerol
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab51460 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution. Predicted molecular weight: 135 kDa.
Target
Function
Acts as a transcriptional repressor. May function in the assembly and/or enzymatic activity of the mSin3A corepressor complex or in mediating interactions between the complex and other regulatory complexes.
Tissue specificity
Expressed in various cancer cell ines.
Sequence similarities
Belongs to the SAP130 family.
Domain
The N-terminus may interact with a transcriptional coactivator.
The C-terminus may interact with HDAC-dependent and HDAC-independent corepressors.
Post-translational
modifications
Acetylated.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ9H0E3
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 130 kDa Sin3-associated polypeptide antibody
- FLJ12761 antibody
- Histone deacetylase complex subunit SAP130 antibody
see all
Database links
- Entrez Gene: 79595 Human
- Omim: 609697 Human
- SwissProt: Q15393 Human
- SwissProt: Q9H0E3 Human
- Unigene: 32995 Human
Anti-SAP130 antibody images:
Western blot - SAP130 antibody (ab51460)
All lanes : Anti-SAP130 antibody (ab51460) at 1 µg/ml
Lane 1 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size : 135 kDa
Observed band size : 135 kDa
Western blot - SAP130 antibody (ab51460)
All lanes : Anti-SAP130 antibody (ab51460)
Lane 1 : 15 µg Hela Nuclear Extract
Lane 2 : 15 µg mouse ES cell nuclear extract
Predicted band size : 135 kDa
References for Anti-SAP130 antibody (ab51460)
ab51460 has not yet been referenced specifically in any publications.
Publishing research using ab51460? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"