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ab30465 |
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ab86702 |
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Dear Sir and/or Madame, have you ever tested the reactivity of the SAP18 antibody "Anti-SAP18 antibody (ab31748)" in the thale cress (Arabidopsis thaliana) although you rule out other potential reactivities for this antibody besides to human? Thanks in advance for your reply. Kind regards, |
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ANSWER: |
Thank you for your inquiry. This antibody is indeed expected to cross react to the following species: Mouse, Rat, Cow, Xenopus laevis and Zebrafish. But this has not yet been experimentally tested. Unfortunately, this antibody's immunogen only shares 2% homology with the sequence for SAP18 from A.thaliana. The antibody therefore will not cross-react with A.thaliana. Please use the following link to review all antibodies for A.thaliana that we offer at the moment: http://www.abcam.com/index.html?pageconfig=resource&rid=11670&viapagetrap=arabidopsis I am sorry I could not give a positive answer on this occasion and hope this information is nevertheless helpful. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
All lanes : Anti-SAP18 antibody (ab31748) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate with
Lysates/proteins at 20 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size : 18 kDa
Observed band size : 21 kDa (why is the actual band size different from the predicted?)
ICC/IF image of ab31748 stained human HeLa cells. The cells were 4% PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab31748, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Image courtesy of Human Protein Atlas
ab31748 stainning SAP18 in paraffin-embedded human cervix, showing a distinct nuclear staining of the surface epithelial (squamous) cells. Tissue sections (4µm) were incubated with ab31748 (1/650 dilution) for 30 minutes at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
ab31748 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org
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