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Products:Epigenetics and Nuclear Signaling >> RNAi >> Argonaute and Piwi
Overview
Product name
Anti-SAP18 antibody
See all SAP18 products (3) ...
Description
Rabbit polyclonal to SAP18
Tested applications
IP, ICC/IF, IHC-P, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Cow, Xenopus laevis, Zebrafish
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human SAP18.
(Peptide available as ab30465.)
Positive control
HeLa (Human epithelial carcinoma cell line) Nuclear Lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS. pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> RNAi >> Argonaute and Piwi
Applications
Show applications key
Our Abpromise guarantee covers the use of ab31748 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
ICC/IF: Use at a concentration of 1 µg/ml.
IHC-P: 1/650. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
IP: Use at a concentration of 1.6 µg/ml (PMID 19503603).
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 18 kDa). Detection of the band is blocked by addition of the immunizing peptide ab30465.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Component of the SIN3-repressing complex. Enhances the ability of SIN3-HDAC1-mediated transcriptional repression. When tethered to the promoter, it can direct the formation of a repressive complex to core histone proteins. Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism.
Tissue specificity
Ubiquitous.
Sequence similarities
Belongs to the SAP18 family.
Cellular localization
Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm.
Target information above from: UniProt accessionO00422
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 18 kDa Sin3-associated polypeptide antibody
- 2HOR0202 antibody
- Cell growth-inhibiting gene 38 protein antibody
see all
Database links
- Entrez Gene: 615692 Cow
- Entrez Gene: 10284 Human
- Entrez Gene: 20220 Mouse
- Entrez Gene: 290284 Rat
- Entrez Gene: 393693 Zebrafish
- Omim: 602949 Human
- SwissProt: Q3T022 Cow
- SwissProt: O00422 Human
see all
Anti-SAP18 antibody images:
Western blot - SAP18 antibody (ab31748)
All lanes : Anti-SAP18 antibody (ab31748) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate with SAP18 peptide (ab30465) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size : 18 kDa
Observed band size : 21 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence - SAP18 antibody (ab31748)
ICC/IF image of ab31748 stained human HeLa cells. The cells were 4% PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab31748, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SAP18 antibody (ab31748)
Image courtesy of Human Protein Atlas
ab31748 stainning SAP18 in paraffin-embedded human cervix, showing a distinct nuclear staining of the surface epithelial (squamous) cells. Tissue sections (4µm) were incubated with ab31748 (1/650 dilution) for 30 minutes at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
ab31748 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org
References for Anti-SAP18 antibody (ab31748)
This product has been referenced in:
- Sorin Met al. Recruitment of a SAP18-HDAC1 complex into HIV-1 virions and its requirement for viral replication. PLoS Pathog 5:e1000463 (2009). WB, IP.Read more (PubMed: 19503603) »
See 1 publication for this product
Publishing research using ab31748? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"