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Products:Epigenetics and Nuclear Signaling >> RNAi >> Argonaute and Piwi
Overview
Product name
SAP18 protein (His tag)
See all SAP18 products (2) ...
Protein description
Recombinant full length human SAP18 fused to His tag at N-terminus; amino acids 1-153 (173 amino acids), 19.7kDa. Swiss ID = O00422
Expression host
E. coli
Properties
Purity
>90% by SDS-PAGE
Purification notes
ab86702 is purified using conventional chromatography techniques. Endotoxin Level: < 1.0 EU per 1 µg of protein (determined by LAL method).
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 30% Glycerol, 0.1M Sodium chloride, 20mM Tris HCl, 1mM DTT, pH 8.0
Concentration
Concentration information loading...
Sequence notes
MGSSHHHHHH SSGLVPRGSH MAVESRVTQE EIKKEPEKPI DREKTCPLLL RVFTTNNGRH HRMDEFSRGN VPSSELQIYT WMDATLKELT SLVKEVYPEA RKKGTHFNFA IVFTDVKRPG YRVKEIGSTM SGRKGTDDSM TLQSQKFQIG DYLDIAITPP NRAPPPSGRM RPY
Research areas
Epigenetics and Nuclear Signaling >> RNAi >> Argonaute and Piwi
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SDS-PAGE - SAP18 protein (His tag) (ab86702)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab86702 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
SDS-PAGE: Use at an assay dependent dilution.
Protein info
Function
Component of the SIN3-repressing complex. Enhances the ability of SIN3-HDAC1-mediated transcriptional repression. When tethered to the promoter, it can direct the formation of a repressive complex to core histone proteins. Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism.
Tissue specificity
Ubiquitous.
Sequence similarities
Belongs to the SAP18 family.
Cellular localization
Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm.
Target information above from: UniProt accessionO00422
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
SAP18 protein (His tag) images:
SDS-PAGE - SAP18 protein (His tag) (ab86702)
15% SDS-PAGE showing ab86702 at approximately 19.7kDa (3µg).
References for SAP18 protein (His tag) (ab86702)
ab86702 has not yet been referenced specifically in any publications.
Publishing research using ab86702? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"