SAP18 protein (His tag) (ab86702)

Product Name 

SAP18 protein (His tag)

Product type 

Proteins and Peptides

Peptide 

Recombinant full length human SAP18 fused to His tag at N-terminus; amino acids 1-153 (173 amino acids), 19.7kDa. Swiss ID = O00422

Tested applications 
(see key)

SDS-PAGE

Application notes 
(see key)

Recommended dilutions
SDS-PAGE: Use at an assay dependent dilution.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Research areas 

Chromatin and Nuclear Signaling >> RNAi >> Argonaute and Piwi

Relevance 

Component of the SIN3-repressing complex. Enhances the ability of SIN3-HDAC1-mediated transcriptional repression. When tethered to the promoter, it can direct the formation of a repressive complex to core histone proteins. Forms a complex with SIN3A and HDAC1.

Purity 

>90% by SDS-PAGE

Storage buffer 

Preservative: None
Constituents: 30% Glycerol, 0.1M Sodium chloride, 20mM Tris HCl, 1mM DTT, pH 8.0

Material safety datasheet (MSDS) for this product:
Glycerol MSDS

Purification notes 

ab86702 is purified using conventional chromatography techniques.
Endotoxin Level: < 1.0 EU per 1 µg of protein (determined by LAL method).

Form 

Liquid

Concentration 

0.500 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Expression system 

E. coli

Sequence 

MGSSHHHHHH SSGLVPRGSH MAVESRVTQE EIKKEPEKPI DREKTCPLLL RVFTTNNGRH HRMDEFSRGN VPSSELQIYT WMDATLKELT SLVKEVYPEA RKKGTHFNFA IVFTDVKRPG YRVKEIGSTM SGRKGTDDSM TLQSQKFQIG DYLDIAITPP NRAPPPSGRM RPY

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