SAP30 antibody (ab15035)

Product Name 

SAP30 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to SAP30

Immunogen 

His-tagged yeast SAP30

Reacts with 
(species key)

Sc

Tested applications 
(see key)

IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
IP: 1 µg immunoprecipitates SAP30 from 500µg of yeast extract.
WB: Use at a concentration of 1 - 4 µg/ml. Detects SAP30 in a yeast extract, proteins can be visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Predicted molecular weight: 27 kDa, however ab15035 crossreacts with numerous additional bands greater than 50kDa in a yeast extract.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Yeast extract

Cellular localization 

Nuclear (probable).

Research areas 

Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HDACs >> Class II / Hda1 Class

Relevance 

Hypoacetylation of core histones is believed to be one mechanism of achieving repression of gene transcription. In human cells, a large multiprotein complex containing the transcriptional repressor Sin3, multiple histone deacetylases (HDAC1 andHDAC2), histone binding proteins (RbAp46 and RbAp48), and other polypeptides, such as SAP30 mediates histone de-acetylation. SAP30 interacts directly with multiple components of this complex, most notably with HDAC1. SAP30 may regulate histone deacetylase activity and substrate specificity. Not all HDAC1-containing complexes contain SAP30 and in yeast Sap30 can recruit the deacetylase complex to promoter DNA in a Sin3 independent manner. Furthermore, SAP30 immunoprecipitates contain reduced deacetylase activity towards histone H3 relative to HDAC1 immunoprecipitates.

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Protein A purified

Storage buffer 

Preservative: 0.035% Sodium Azide
Constituents: 30% Glycerol, 0.105M Sodium chloride, 0.07M Tris glycine. pH 7.2

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Customer reviews (feedback) regarding SAP30 antibody

Customer FAQs regarding SAP30 antibody

Protocols for SAP30 antibody

Price and availability of products related to SAP30 antibody

SAP30 antibody - related products: