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Western blot - SAP30 antibody (ab56527)
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Product Name
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SAP30 antibody
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See all SAP30 antibodies (5)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal to SAP30
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Immunogen
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Recombinant fragment: SDDDGGDSPV QDIDTPEVDL YQLQVNTLRR YKRHFKLPTR PGLNKAQLVE IVGCHFRSIP VNEKDTLTYF IYSVKNDKNK SDLKVDSGV, corresponding to amino acids 131-220 of Human SAP30
BLAST 'SDDDGGDSPV QDIDTPEVDL YQLQVNTLRR YKRHFKLPTR PGLNKAQLVE IVGCHFRSIP VNEKDTLTYF IYSVKNDKNK ' with  or 
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Reacts with
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Hu
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Tested applications
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WB
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Abreviews
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Application notes
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Recommended dilutions
WB: Use at a concentration of 1-5 µg/ml.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear (probable).
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Research areas
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Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HDACs >> Class II / Hda1 Class
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Relevance
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Hypoacetylation of core histones is believed to be one mechanism of achieving repression of gene transcription. In human cells, a large multiprotein complex containing the transcriptional repressor Sin3, multiple histone deacetylases (HDAC1 andHDAC2), histone binding proteins (RbAp46 and RbAp48), and other polypeptides, such as SAP30 mediates histone de-acetylation. SAP30 interacts directly with multiple components of this complex, most notably with HDAC1. SAP30 may regulate histone deacetylase activity and substrate specificity. Not all HDAC1-containing complexes contain SAP30 and in yeast Sap30 can recruit the deacetylase complex to promoter DNA in a Sin3 independent manner. Furthermore, SAP30 immunoprecipitates contain reduced deacetylase activity towards histone H3 relative to HDAC1 immunoprecipitates.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Isotype
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IgG2b
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Light chain type
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kappa
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Purity
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Protein G purified
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Storage buffer
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Preservative: None
PBS, pH 7.2
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this SAP30 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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SAP30 antibody images:
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 Western blot - SAP30 antibody (ab56527)
Western blot against tagged recombinant protein immunogen using ab56527 SAP30 antibody at 1ug/ml. Predicted band size of immunogen is 36 kDa
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Search PubMed (MEDLINE) for references to SAP30
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SAP30 antibody - more information
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Customer reviews (feedback) regarding SAP30 antibody |
Customer FAQs regarding SAP30 antibody |
Protocols for SAP30 antibody |
Price and availability of products related to SAP30 antibody |
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SAP30 antibody - other tools:
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Contact Abcam with a Technical Enquiry about SAP30 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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