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Overview
Product name
Anti-SAP30 antibody
See all SAP30 products (4) ...
Description
Mouse monoclonal to SAP30
Tested applications
IHC-P, ICC/IF, ELISA, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant fragment: SDDDGGDSPV QDIDTPEVDL YQLQVNTLRR YKRHFKLPTR PGLNKAQLVE IVGCHFRSIP VNEKDTLTYF IYSVKNDKNK SDLKVDSGV, corresponding to amino acids 131-220 of Human SAP30
SDDDGGDSPV QDIDTPEVDL YQLQVNTLRR YKRHFKLPTR PGLNKAQLVE IVGCHFRSIP VNEKDTLTYF IYSVKNDKNK SDLKVDSGV
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Isotype
IgG2b
Light chain type
kappa
Applications
Show applications key
Our Abpromise guarantee covers the use of ab56527 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use a concentration of 1.5 µg/ml(Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.)
ICC/IF: Use a concentration of 10 µg/ml
ELISA: Use at an assay dependent concentration. (Detection limit for recombinant GST tagged SAP30 is approximately 1ng/ml.)
WB: Use a concentration of 1 - 5 µg/ml.(This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.)
Target
Function
Involved in the functional recruitment of the Sin3-histone deacetylase complex (HDAC) to a specific subset of N-CoR corepressor complexes. Capable of transcription repression by N-CoR. Active in deacetylating core histone octamers (when in a complex) but inactive in deacetylating nucleosomal histones.
Tissue specificity
Expressed in all tissues tested with highest levels in pancreas, ovary, PBL, spleen and thymus; lowest levels in brain, placenta, lung and kidney.
Sequence similarities
Belongs to the SAP30 family.
Cellular localization
Nucleus.
Target information above from: UniProt accessionO75446
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 30 kDa Sin3-associated polypeptide antibody
- Histone deacetylase complex subunit SAP 30 antibody
- Histone deacetylase complex subunit SAP30 antibody
see all
Database links
- Entrez Gene: 8819 Human
- Omim: 603378 Human
- SwissProt: O75446 Human
- Unigene: 512813 Human
- Unigene: 591715 Human
Anti-SAP30 antibody images:
Western blot - Anti-SAP30 antibody (ab56527)
Western blot against tagged recombinant protein immunogen using ab56527 SAP30 antibody at 1ug/ml. Predicted band size of immunogen is 36 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAP30 antibody (ab56527)
Immunoperoxidase of monoclonal antibody to SAP30 on formalin-fixed paraffin-embedded Human esophagus. [antibody concentration 1.5 ug/ml]
Immunocytochemistry/ Immunofluorescence - Anti-SAP30 antibody (ab56527)
Immunofluorescence of monoclonal antibody to SAP30 on HeLa cell . [antibody concentration 10 ug/ml]
References for Anti-SAP30 antibody (ab56527)
ab56527 has not yet been referenced specifically in any publications.
Publishing research using ab56527? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"