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Overview
Product name
Anti-SAP30 antibody
See all SAP30 products (3) ...
Description
Rabbit polyclonal to SAP30
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Chicken, Cow, Dog
Immunogen
A region within synthetic peptide: PEVDLYQLQV NTLRRYKRHF KLPTRPGLNK AQLVEIVGCH FRSIPVNEKD, corresponding to internal sequence amino acids 146-195 of Human SAP30
PEVDLYQLQV NTLRRYKRHF KLPTRPGLNK AQLVEIVGCH FRSIPVNEKD
Positive control
293T cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
-
Western blot - SAP30 antibody (ab65559)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab65559 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1.25 µg/mlDetects a band of approximately 23 kDa (predicted molecular weight: 26 kDa).
Target
Function
Involved in the functional recruitment of the Sin3-histone deacetylase complex (HDAC) to a specific subset of N-CoR corepressor complexes. Capable of transcription repression by N-CoR. Active in deacetylating core histone octamers (when in a complex) but inactive in deacetylating nucleosomal histones.
Tissue specificity
Expressed in all tissues tested with highest levels in pancreas, ovary, PBL, spleen and thymus; lowest levels in brain, placenta, lung and kidney.
Sequence similarities
Belongs to the SAP30 family.
Cellular localization
Nucleus.
Target information above from: UniProt accessionO75446
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 30 kDa Sin3-associated polypeptide antibody
- Histone deacetylase complex subunit SAP 30 antibody
- Histone deacetylase complex subunit SAP30 antibody
see all
Database links
- Entrez Gene: 8819 Human
- Entrez Gene: 60406 Mouse
- Omim: 603378 Human
- SwissProt: O75446 Human
- SwissProt: O88574 Mouse
- Unigene: 512813 Human
- Unigene: 591715 Human
- Unigene: 118 Mouse
Anti-SAP30 antibody images:
Western blot - SAP30 antibody (ab65559)
Anti-SAP30 antibody (ab65559) at 1.25 µg/ml + transfected 293T cell lysate
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 26 kDa
Observed band size : 23 kDa (why is the actual band size different from the predicted?)
Ab65559 diluted in 5% skim milk / PBS buffer.
References for Anti-SAP30 antibody (ab65559)
ab65559 has not yet been referenced specifically in any publications.
Publishing research using ab65559? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"