Anti-SAP30L antibody - C-terminal (ab220491)
Key features and details
- Rabbit polyclonal to SAP30L - C-terminal
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SAP30L antibody - C-terminal -
Description
Rabbit polyclonal to SAP30L - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Xenopus laevis, Xenopus tropicalis -
Immunogen
Recombinant fragment corresponding to Human SAP30L aa 150 to the C-terminus (C terminal).
Database link: Q9HAJ7 -
Positive control
- WB: RT4, U251 MG cell lysate, human liver and tonsil tissue lysates. ICC/IF: MCF7 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab220491 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 21 kDa.
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
Notes |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 21 kDa. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
Target
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Function
Involved in the functional recruitment of the class 1 Sin3-histone deacetylase complex (HDAC) to the nucleolus. -
Tissue specificity
Expressed in all tissues tested with highest levels in testis. -
Sequence similarities
Belongs to the SAP30 family. -
Cellular localization
Nucleus > nucleolus. - Information by UniProt
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Database links
- Entrez Gene: 79685 Human
- Entrez Gene: 50724 Mouse
- Entrez Gene: 380449 Xenopus laevis
- Entrez Gene: 735024 Xenopus laevis
- Entrez Gene: 549895 Xenopus tropicalis
- Omim: 610398 Human
- SwissProt: Q9HAJ7 Human
- SwissProt: Q5SQF8 Mouse
see all -
Alternative names
- FLJ11526 antibody
- FLJ23595 antibody
- FLJ36497 antibody
see all
Images
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Immunofluorescent analysis of MCF7 cells (PFA-fixed/Triton X-100 permeabilized) labeling SAP30L with ab220491 at 4 μg/mL (green).
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All lanes : Anti-SAP30L antibody - C-terminal (ab220491) at 1/100 dilution
Lane 1 : RT4 cell lysate
Lane 2 : U251 MG cell lysate
Lane 3 : Human plasma lysate
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Predicted band size: 21 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab220491 has not yet been referenced specifically in any publications.