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Products:Cell Biology >> Other Antibodies >> Other Antibodies
Overview
Product name
Anti-SAP30L antibody
See all SAP30L products (2) ...
Description
Rabbit polyclonal to SAP30L
Specificity
Detects wild-type transfected hSAP30L or GST-hSAP30L fusion protein as well as endogenous hSAP30L in human cell lines (e.g., HEK293 and MCF-7 cells). Detects wild-type transfected hSAP30L or GST-hSAP30L fusion protein in non-human cell lines (e.g., NIH3T3 cells), but does not detect endogenous non-human SAP30L.
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse
Immunogen
Synthetic peptide: VKSNKSRLDQKSEGGKQLE, corresponding to amino acids 165-183 of Human SAP30L
VKSNKSRLDQ KSEGGKQLE
Positive control
HEK 293 cell lysate MCF 7 cell lysate
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HDACs >> Class I
Cell Biology >> Other Antibodies >> Other Antibodies
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Western blot - SAP30L antibody (ab33244)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab33244 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: 1/500 - 1/1000. Detects a band of approximately 21-22 kDa (predicted molecular weight: 21 kDa).
The transfected wild-type hSAP30L runs with an apparent molecular weight of ~30kDa and the endogenous hSAP30L band runs with an apparent molecular weight of 21-22 kDa. The difference in the size of the bands is unknown, but is seen by investigators in all studies of hSAP30L. Possible explanations for the size difference may be to cleavage product, degradation and/or product or post-translational modification.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
SAP30L has been identified as a novel TGF-beta up-regulated mRNA species, the Sin3-associated protein 30-like. The predicted nuclear localization signal of SAP30L is sufficient for nuclear transport of the protein although mutating it does not completely remove SAP30L from the nuclei. It is thought that SAP30L might have a role in recruiting the Sin3-histone deacetylase complex to specific co-repressor complexes in response to TGF-beta, leading to the silencing of proliferation-driving genes in the differentiating intestinal epithelial cells, due to its nuclear localization and close homology to SAP30.
Cellular localization
Nuclear
Alternative names
- FLJ11526 antibody
- FLJ23595 antibody
- FLJ36497 antibody
see all
Database links
- Entrez Gene: 79685 Human
- Entrez Gene: 50724 Mouse
- SwissProt: Q9HAJ7 Human
- Unigene: 483906 Human
Anti-SAP30L antibody images:
Western blot - SAP30L antibody (ab33244)
All lanes : Anti-SAP30L antibody (ab33244) at 1/750 dilution
Lane 1 : 30ug of NIH3T3 cell lysate transfected with a vector expressing a GST-hSAP30L fusion protein
Lane 2 : 30ug HEK293 cell lysate expressing both a Wild type transfected hSAP30L and endogenous SAP30L
Lane 3 : 30ug MCF-7 cell lysate
Predicted band size : 21 kDa
ab33244 is able to detect GST-SAP30L fusion protein, wild -type transfected hSAP30L and endogenous SAP30L in human cell lines (eg: HEK293 and MCF-7); Can also detect GST-SAP30L and Wild-Type transfected hSAP30L in non-human cell lines but it does not seem to be able to detect endogenous, non-human SAP30L.
References for Anti-SAP30L antibody (ab33244)
ab33244 has not yet been referenced specifically in any publications.
Publishing research using ab33244? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"