SAP30L antibody (ab33244)

Product Name 

SAP30L antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to SAP30L

Immunogen 

Synthetic peptide: VKSNKSRLDQKSEGGKQLE, corresponding to amino acids 165-183 of Human SAP30L
BLAST 'VKSNKSRLDQKSEGGKQLE' with or

Reacts with 
(species key)

Hu

Specificity 

Detects wild-type transfected hSAP30L or GST-hSAP30L fusion protein as well as endogenous hSAP30L in human cell lines (e.g., HEK293 and MCF-7 cells). Detects wild-type transfected hSAP30L or GST-hSAP30L fusion protein in non-human cell lines (e.g., NIH3T3 cells), but does not detect endogenous non-human SAP30L.

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: 1/500 - 1/1000. Detects a band of approximately 21-22 kDa (predicted molecular weight: 21 kDa).

The transfected wild-type hSAP30L runs with an apparent molecular weight of ~30kDa and the endogenous hSAP30L band runs with an apparent molecular weight of 21-22 kDa. The difference in the size of the bands is unknown, but is seen by investigators in all studies of hSAP30L. Possible explanations for the size difference may be to cleavage product, degradation and/or product or post-translational modification.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

HEK 293 cell lysate
MCF 7 cell lysate

Cellular localization 

Nuclear

Research areas 

Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HDACs >> Class I
Cell Biology >> Other Antibodies >> Other Antibodies

Relevance 

SAP30L has been identified as a novel TGF-beta up-regulated mRNA species, the Sin3-associated protein 30-like. The predicted nuclear localization signal of SAP30L is sufficient for nuclear transport of the protein although mutating it does not completely remove SAP30L from the nuclei. It is thought that SAP30L might have a role in recruiting the Sin3-histone deacetylase complex to specific co-repressor complexes in response to TGF-beta, leading to the silencing of proliferation-driving genes in the differentiating intestinal epithelial cells, due to its nuclear localization and close homology to SAP30.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: PBS

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

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