Overview

  • Product nameAnti-SAP97 antibody
    See all SAP97 primary antibodies
  • Description
    Rabbit polyclonal to SAP97
  • SpecificityBy Western blot, this antibody detects an ~140 kDa protein representing SAP 97 from rat brain extract. Immunohistochemical staining of SAP 97 in rat hippocampus results in intense neuronal cell body and dendrite staining. No cross-reactivity to other proteins, including synapse-associated protein family members, has been observed.
  • Tested applicationsICC/IF, ICC, IP, IHC-Fr, WB, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Rat SAP97 aa 115-133.
    Sequence:

    VLPSERISPQVPNEVLGPE


    (Peptide available as ab5852)

  • Positive control
    • WB: rat brain extract IHC: rat hippocampus

Properties

Applications

Our Abpromise guarantee covers the use of ab3437 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/20 - 1/200.
ICC 1/20 - 1/200.
IP Use at an assay dependent concentration.
IHC-Fr 1/1000.
WB 1/500 - 1/5000. Detects a band of approximately 140 kDa (predicted molecular weight: 140 kDa).Can be blocked with SAP97 peptide (ab5852).
IHC-FoFr 1/3000.

Target

  • FunctionEssential multidomain scaffolding protein required for normal development (By similarity). Recruits channels, receptors and signaling molecules to discrete plasma membrane domains in polarized cells. May play a role in adherens junction assembly, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. Regulates the excitability of cardiac myocytes by modulating the functional expression of Kv4 channels. Functional regulator of Kv1.5 channel.
  • Tissue specificityAbundantly expressed in atrial myocardium (at protein level). Expressed in lung fibroblasts, cervical epithelial and B-cells (at protein level). Widely expressed, with isoforms displaying different expression profiles.
  • Sequence similaritiesBelongs to the MAGUK family.
    Contains 1 guanylate kinase-like domain.
    Contains 1 L27 domain.
    Contains 3 PDZ (DHR) domains.
    Contains 1 SH3 domain.
  • DomainThe alternatively spliced domain I3 corresponding to amino acids (636-669) of isoform 4 is an EPB41 binding site mediating association to membranes in polarized and non-polarized cells.
    The PDZ domains may also mediate association to membranes by binding to EPB41 and GPR124 together with the L27 domain that binds CASK and DLG2.
    The L27 domain may regulate DLG1 self-association. The N-terminal alternatively spliced region is capable of binding several SH3 domains and also moderates the level of protein oligomerization.
  • Post-translational
    modifications
    Phosphorylated by MAPK12. Phosphorylation of Ser-232 regulates association with GRIN2A. Isoform 2 is phosphorylated on Ser-698. Isoform 3 is phosphorylated on Ser-665.
  • Cellular localizationMembrane. Basolateral cell membrane. Endoplasmic reticulum membrane. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cell junction > synapse. Cell membrane > sarcolemma. Colocalizes with EPB41 at regions of intercellular contacts. Basolateral in epithelial cells. May also associate with endoplasmic reticulum membranes. Mainly found in neurons soma, moderately found at postsynaptic densities.
  • Information by UniProt
  • Database links
  • Alternative names
    • Discs large homolog 1 antibody
    • discs large, Drosophila, homolog of, 1 antibody
    • discs, large homolog 1 (Drosophila) antibody
    • Disks large homolog 1 antibody
    • dJ1061C18.1.1 antibody
    • DKFZp761P0818 antibody
    • DKFZp781B0426 antibody
    • DLG 1 antibody
    • DLG1 antibody
    • DLG1_HUMAN antibody
    • DLGH 1 antibody
    • DLGH1 antibody
    • HDLG antibody
    • Presynaptic protein SAP97 antibody
    • SAP 97 antibody
    • SAP-97 antibody
    • SAP97 antibody
    • Synapse associated protein 97 antibody
    • Synapse-associated protein 97 antibody
    see all

Anti-SAP97 antibody images

  • Immunocytochemistry/Immunofluorescent analysis of SAP97 (green) showing staining in the cytoplasm and membrane of NIH-3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab3437 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 �C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
  • Immunocytochemistry/Immunofluorescent analysis of SAP97 (green) showing staining in the cytoplasm and membrane of C2C12 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab3437 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 �C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
  • Immunocytochemistry/Immunofluorescent analysis of SAP97 (green) showing staining in the cytoplasm and membrane of HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab3437 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 �C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
  • Immunofluorescent staining in the rat cortex with ab3437, rabbit polyclonal antibody to rat SAP97. Picture taken with a X40 objective. Protocol: IHC free-floating protocol using 4% PFA fixed brain tissue. Rats were intracardially perfused with 4% PFA. Tissue was post-fixed overnight in the same fixative, cryoprotected in 20% sucrose and frozen in OCT. Primary antibody ab3437 was used at 1/3000 incubated overnight at room temperature. Secondary antibody was Alexa Fluor 488 used at 1/1000, 2h incubation at room temperature. Image recoloured in Adobe photoshop.
  • ICC/IF image of ab3437 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3437, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-SAP97 antibody (ab3437) at 1/5000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : PC12 cell lysate
    Lane 3 : Rat brain cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 140 kDa

References for Anti-SAP97 antibody (ab3437)

This product has been referenced in:
  • Dolmatova E  et al. Cardiomyocyte ATP release through pannexin 1 aids in early fibroblast activation. Am J Physiol Heart Circ Physiol 303:H1208-18 (2012). Read more (PubMed: 22982782) »
  • Villa L  et al. Effects and mechanisms of angiotensin II receptor blockade with telmisartan in a normotensive model of mesangioproliferative nephritis. Nephrol Dial Transplant 26:3131-43 (2011). Read more (PubMed: 21414972) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Recombinant protein (HEK293)
Loading amount 50 µg
Specification HEK293
Gel Running Conditions Reduced Denaturing (7.5%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Abcam user community

Verified customer

Submitted Apr 27 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Recombinant protein (Transfected Cells)
Loading amount 50 µg
Specification Transfected Cells
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Mar 17 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (Brain sections (30 um free floating))
Specification Brain sections (30 um free floating)
Fixative Paraformaldehyde
Permeabilization No
Username

Dr. Sophie Pezet

Verified customer

Submitted Dec 06 2007

Unfortunately we have not determined the concentration nor the exact isotype beyond IgG of this antibody.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"