Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Related products
- Secondary antibodies
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Overview
Product name
Anti-SAPK4 antibody
See all SAPK4 products (5) ...
Description
Rabbit polyclonal to SAPK4
Specificity
ab37936 recognises human, mouse, rat and primate SAPK4.
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Predicted to work with
Non Human Primates
Immunogen
KLH conjugated synthetic peptide, selected within C terminal amino acids 350-400 of human SAPK4.
Positive control
HCT116 and PAM212 cell lysates.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
Eluted out with both high and low pH buffers and neutralized immediately after elution, followed by dialysis against PBS.
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> MAPK Pathway
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Other
-
Western blot - SAPK4 antibody (ab37936)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab37936 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/1000.ELISA: 1/1000.
WB: 1/100 - 1/500. Predicted molecular weight: 42 kDa.
Target
Function
Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating downstream targets. Plays a role in the regulation of protein translation by phosphorylating and inactivating EEF2K.
Tissue specificity
Expressed in testes, pancreas, small intestine, lung and kidney. Abundant in macrophages, also present in neutrophils, CD4+ T-cells, and endothelial cells.
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain.
Domain
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
Post-translational
modifications
Dually phosphorylated on Thr-180 and Tyr-182, which activates the enzyme.
Target information above from: UniProt accessionO15264
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- MAP kinase 13 antibody
- MAP kinase p38 delta antibody
- MAPK 13 antibody
see all
Database links
- Entrez Gene: 5603 Human
- Entrez Gene: 26415 Mouse
- Entrez Gene: 29513 Rat
- Omim: 602899 Human
- SwissProt: O15264 Human
- SwissProt: Q9Z1B7 Mouse
- SwissProt: Q9WTY9 Rat
- SwissProt: Q9WTY9 Rat
see all
Anti-SAPK4 antibody images:
Western blot - SAPK4 antibody (ab37936)
All lanes : Anti-SAPK4 antibody (ab37936) at 1/100 dilution
Lane 1 : nocodazole treated HCT116 cell lysate
Lane 2 : PMA-treated Pam212 cell lysate
Predicted band size : 42 kDa
Observed band size : 42 kDa
References for Anti-SAPK4 antibody (ab37936)
This product has been referenced in:
- Neidhart M et al. Trex-1 deficiency in rheumatoid arthritis synovial fibroblasts. Arthritis Rheum 62:2673-9 (2010). WB; Human.Read more (PubMed: 20496420) »
See 1 publication for this product
Publishing research using ab37936? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"