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If your product does not perform as described on this datasheet, we will refund or replace your product...
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Western blot - SAPS3 antibody (ab72034)
All lanes : Anti-SAPS3 antibody (ab72034) at 0.02 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 50 µg
Lane 2 : Whole cell lysate from Hela cells at 15 µg
Lane 3 : Whole cell lysate from Hela cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Predicted band size : 98 kDa
Observed band size : 120 kDa (why is the actual band size different from the predicted?)
Immunoprecipitation - SAPS3 antibody (ab72034)
Immunoprecipitation/ Western Blot of SAPS3.
Lane 1: For IP: ab72034 at 3µg/mg whole cell lysate, with ab72034 at 0.04µg/ml for subsequent WB.
Lane 2: Control IgG.
Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
Chemiluminescence with an exposure time of 3 seconds.
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